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Literature DB >> 5449121

Measurement of oxidized glutathione and total glutathione in the perfused rat heart.

Abstract

1. A method was developed for the assay of GSSG in heart tissue. 2. GSSG and total glutathione were measured in rat hearts perfused under a variety of conditions. About 2% of the total glutathione is present as GSSG. The concentrations of GSSG and GSH remained constant under all the conditions tested. 3. These results are discussed with reference to the equilibrium and rate of the glutathione reductase reaction in the cell. It is concluded that the enzyme reaction does not lie near equilibrium.

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Year: 1970 PMID： 5449121 PMCID： PMC1179016 DOI： 10.1042/bj1170661

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. EFFECT OF INSULIN ON THE LEVELS OF OXIDIZED AND REDUCED NICOTINAMIDE-ADENINE DINUCLEOTIDES IN THE PERFUSED RAT HEART.

Authors: J R WILLIAMSON; R A KREISBERG
Journal: Biochim Biophys Acta Date: 1965-02-15

2. [A simple technic for extremely rapid freezing of large pieces of tissue].

Authors: A WOLLENBERGER; O RISTAU; G SCHOFFA
Journal: Pflugers Arch Gesamte Physiol Menschen Tiere Date: 1960

3. Glutathione, oxidized and reduced, in the brain and in isolated cerebral tissue.

Authors: H MARTIN; H McILWAIN
Journal: Biochem J Date: 1959-02 Impact factor: 3.857

4. Regulation of glucose uptake in muscle. I. The effects of insulin and anoxia on glucose transport and phosphorylation in the isolated, perfused heart of normal rats.

Authors: H E MORGAN; M J HENDERSON; D M REGEN; C R PARK
Journal: J Biol Chem Date: 1961-02 Impact factor: 5.157

5. The reduction of oxidized glutathione in erythrocyte haemolysates in pernicious anaemia.

Authors: P C JOCELYN
Journal: Biochem J Date: 1960-11 Impact factor: 3.857

6. REDUCTION-POTENTIAL OF GLUTATHIONE.

Authors: J ROST; S RAPOPORT
Journal: Nature Date: 1964-01-11 Impact factor: 49.962

7. Glutathione reductase of animal tissues.

Authors: T W RALL; A L LEHNINGER
Journal: J Biol Chem Date: 1952-01 Impact factor: 5.157

8. Regulation of glucose uptake by muscles. 10. Effects of alloxan-diabetes, starvation, hypophysectomy and adrenalectomy, and of fatty acids, ketone bodies and pyruvate, on the glycerol output and concentrations of free fatty acids, long-chain fatty acyl-coenzyme A, glycerol phosphate and citrate-cycle intermediates in rat heart and diaphragm muscles.

Authors: P B Garland; P J Randle
Journal: Biochem J Date: 1964-12 Impact factor: 3.857

9. The true oxidized glutathione content of red blood cells obtained by new enzymic and paper chromatographic methods.

Authors: H Güntherberg; J Rost
Journal: Anal Biochem Date: 1966-05 Impact factor: 3.365

10. The redox state of free nicotinamide-adenine dinucleotide phosphate in the cytoplasm of rat liver.

Authors: R L Veech; L V Eggleston; H A Krebs
Journal: Biochem J Date: 1969-12 Impact factor: 3.857

11 in total

1. Regulation of the oxidative phase of the pentose phosphate cycle in mussels.

Authors: S Rodriguez-Segade; M Freire; A Carrion
Journal: Biochem J Date: 1978-03-15 Impact factor: 3.857

Review 2. Stimulability, adenosine triphosphatases and their control by cellular redox processes.

Authors: S Dikstein
Journal: Naturwissenschaften Date: 1971-09

3. Thiol-dependent changes in the properties of rat liver sulphotransferases.

Authors: D J Barford; J G Jones
Journal: Biochem J Date: 1971-07 Impact factor: 3.857

4. Glutathione contents of tissues in the aging mouse.

Authors: G A Hazelton; C A Lang
Journal: Biochem J Date: 1980-04-15 Impact factor: 3.857

5. Regulation of the pentose phosphate cycle.

Authors: L V Eggleston; H A Krebs
Journal: Biochem J Date: 1974-03 Impact factor: 3.857

6. Determination of aliphatic thiols by fluorometric high-performance liquid chromatography after precolumn derivatization with 2-(4-N-maleimidophenyl)-6-methylbenzothiazole.

Authors: A I Haj-Yehia; L Z Benet
Journal: Pharm Res Date: 1995-01 Impact factor: 4.200