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Literature DB >> 5439088

An automated method for the measurement of folate activity.

Abstract

A new approach to the estimation of folate activity is described. The system may be used as a semi-automated method or as a fully automated method using the AutoAnalyzer or Mecolab equipment. The methods use a chloramphenicol-resistant strain of L. casei as the test organism, and this eliminates the need for sterilization or aseptic addition. The systems are able to handle extremely large numbers of samples in a relatively short time. Results are similar to those obtained by standard techniques.

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Year: 1970 PMID： 5439088 PMCID： PMC474411 DOI： 10.1136/jcp.23.1.47

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

9 in total

1. A microbiologic method for detecting folic acid deficiency in man.

Authors: H BAKER; V HERBERT; O FRANK; I PASHER; S H HUTNER; L R WASSERMAN; H SOBOTKA
Journal: Clin Chem Date: 1959-08 Impact factor: 8.327

2. The microbiological assay of serum folic acid activity.

Authors: R E DAVIS; A KELLY
Journal: Aust J Exp Biol Med Sci Date: 1962-12

3. Observations on the metabolism of folic acid in pernicious anaemia.

Authors: A H WATERS; D L MOLLIN
Journal: Br J Haematol Date: 1963-07 Impact factor: 6.998

4. The assay and nature of folic acid activity in human serum.

Authors: V HERBERT
Journal: J Clin Invest Date: 1961-01 Impact factor: 14.808

5. Differential stability of various analogs of cobalamin to vitamin C.

Authors: D V FROST; M LAPIDUS; K A PLAUT; E SCHERFLING; H H FRICKE
Journal: Science Date: 1952-08-01 Impact factor: 47.728

6. Microbiological assay of serum and whole-blood folic acid activity.

Authors: J M Cooperman
Journal: Am J Clin Nutr Date: 1967-09 Impact factor: 7.045

7. A modified 'aseptic addition' assay procedure for the measurement of serum 'folic-acid' activity.

Authors: T A Harper
Journal: Nature Date: 1965-08-28 Impact factor: 49.962

8. Aseptic addition method for Lactobacillus casei assay of folate activity in human serum.

Authors: V Herbert
Journal: J Clin Pathol Date: 1966-01 Impact factor: 3.411

9. Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency.

Authors: A V Hoffbrand; F A Newcombe; D L Mollin
Journal: J Clin Pathol Date: 1966-01 Impact factor: 3.411

9 in total

22 in total

An automated method for the measurement of folate activity.

1. A microbiologic method for detecting folic acid deficiency in man.

2. The microbiological assay of serum folic acid activity.

3. Observations on the metabolism of folic acid in pernicious anaemia.

4. The assay and nature of folic acid activity in human serum.

5. Differential stability of various analogs of cobalamin to vitamin C.

6. Microbiological assay of serum and whole-blood folic acid activity.

7. A modified 'aseptic addition' assay procedure for the measurement of serum 'folic-acid' activity.

8. Aseptic addition method for Lactobacillus casei assay of folate activity in human serum.

9. Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency.

1. Serum pyridoxal in patients with gastric pathology.

2. Serum pyridoxal in patients with rheumatoid arthritis.

3. Comparison of serum and red blood cell folate microbiologic assays for national population surveys.

4. An explanation of the decreased serum folate values found using methods that involve prior extraction of the serum.

5. A comparison of serum folate estimations using two different methods.

6. Experience with microbiological assay for folate using a chloramphenicol-resistant L. casei strain.

7. Use of the 'Analmatic clinical system' in the microbiological assay of vitamin B 12 and folic acid in serum.

8. A regional quality control trial of folate assays.

9. An automated microbiological method for the measurement of vitamin B12.

10. Serum pyridoxal in active peptic ulceration.