Literature DB >> 53784

DNA-synthesis on giant nuclear RNA by AMV DNA polymerase.

V M Kavsan, A V Rynditch, O P Samarina, G P Georgiev.   

Abstract

The reverse transcription of pre-mRNA isolated from rat liver or mouse Ehrlich ascites carcinoma cells with the aid of hot phenol fractionation technique is described. Pre-mRNA isolated at 85 degrees C is a more active template than the 65 degree C fraction. The addition of oligo(dT) as a primer strongly stimulated the template activity of the 65 degree C fraction. The size of product corresponds to a sedimentation value of 7 S as measured in alkaline sucrose gradient and is essentially less than the size of template.

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Year:  1975        PMID： 53784     DOI： 10.1007/bf00356989

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  9 in total

1.  In vitro synthesis of DNA components of human genes for globins.

Authors:  A Bank; M Terada; S Metafora; L Dow; P A Marks
Journal:  Nat New Biol       Date:  1972-02-09

2.  In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

Authors:  I M Verma; G F Temple; H Fan; D Baltimore
Journal:  Nat New Biol       Date:  1972-02-09

3.  Conditions for the selective synthesis of DNA complementary to template RNA.

Authors:  R M Ruprecht; N C Goodman; S Spiegelman
Journal:  Biochim Biophys Acta       Date:  1973-01-19

4.  Isolation of poly(adenylic acid)-rich ribonucleic acid from mouse myeloma and synthesis of complementary deoxyribonucleic acid.

Authors:  C H Faust; H Diggelmann; B Mach
Journal:  Biochemistry       Date:  1973-02-27       Impact factor: 3.162

5.  Evidence for 30-40S RNA as precursor of the 60-70S RNA of Rous sarcoma virus.

Authors:  E Canaani; K V Helm; P Duesberg
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

6.  On the structural organization of the transcriptional unit in animal chromosomes.

Authors:  G P Georgiev; A J Varshavsky; A P Ryskov; R B Church
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

7.  Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells.

Authors:  G R Molloy; W L Thomas; J E Darnell
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

8.  Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid.

Authors:  D S Holmes; J Bonner
Journal:  Biochemistry       Date:  1973-06-05       Impact factor: 3.162

9.  Synthesis of a deoxyribonucleic acid sequence complementary to ovalbumin messenger ribonucleic acid and quantification of ovalbumin genes.

Authors:  D Sullivan; R Palacios; J Stavnezer; J M Taylor; A J Faras; M L Kiely; N M Summers; J M Bishop; R T Schimke
Journal:  J Biol Chem       Date:  1973-11-10       Impact factor: 5.157
  9 in total
  1 in total

1.  Mechanism of linear DNA circularization: formation of 'lasso'-like structures of pre-mRNA DNA copies.

Authors:  V M Kavsan; A V Rynditch; A D Shved
Journal:  Mol Biol Rep       Date:  1982-04-16       Impact factor: 2.316
  1 in total

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