Literature DB >> 5378381

Phosphate-stimulated breakdown of 5'-methylthioadenosine by rat ventral prostate.

A E Pegg, H G Williams-Ashman.   

Abstract

A soluble enzyme preparation catalysing the release of adenine from 5'-methylthioadenosine was purified some 30-fold from extracts of the rat ventral prostate. This reaction was completely dependent on addition of inorganic phosphate ions to the assay medium. This absolute requirement for phosphate ions suggests a phosphorolytic cleavage mechanism. After acid treatment, the other product of the reaction appeared to be 5-methylthioribose. The actions of some other well-characterized enzymes of nucleoside metabolism of 5'-methylthioadenosine were also investigated; purified purine nucleoside phosphorylases from calf spleen and human erythrocytes did not attack 5'-methylthioadenosine. The role of 5'-methylthioadenosine in mammalian tissues is discussed.

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Year:  1969        PMID： 5378381      PMCID： PMC1185095          DOI： 10.1042/bj1150241

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  The enzymic oxidation and assay of adenine.

Authors:  H KLENOW
Journal:  Biochem J       Date:  1952-01       Impact factor: 3.857

3.  On the role of S-adenosyl-L-methionine in the biosynthesis of spermidine by rat prostate.

Authors:  A E Pegg; H G Williams-Ashman
Journal:  J Biol Chem       Date:  1969-02-25       Impact factor: 5.157

4.  Purine nucleoside phosphorylase from human erythrocytes. I. Purification and properties.

Authors:  B K Kim; S Cha; R E Parks
Journal:  J Biol Chem       Date:  1968-04-25       Impact factor: 5.157

5.  Inhibition of human purine nucleoside phosphorylase. Studies with intact erythrocytes and the purified enzyme.

Authors:  T A Krenitsky; G B Elion; A M Henderson; G H Hitchings
Journal:  J Biol Chem       Date:  1968-06-10       Impact factor: 5.157

6.  Purine nucleoside phosphorylase from human erythroyctes. II. Kinetic analysis and substrate-binding studies.

Authors:  B K Kim; S Cha; R E Parks
Journal:  J Biol Chem       Date:  1968-04-25       Impact factor: 5.157

7.  Stimulation of the decarboxylation of S-adenosylmethionine by putrescine in mammalian tissues.

Authors:  A E Pegg; H G Williams-Ashman
Journal:  Biochem Biophys Res Commun       Date:  1968-01-11       Impact factor: 3.575

8.  Biosynthesis of putrescine in the prostate gland of the rat.

Authors:  A E Pegg; H G Williams-Ashman
Journal:  Biochem J       Date:  1968-07       Impact factor: 3.857

9.  Purine nucleoside phosphorylase from human erythrocytes. IV. Crystallization and some properties.

Authors:  R P Agarwal; R E Parks
Journal:  J Biol Chem       Date:  1969-02-25       Impact factor: 5.157

10.  The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. X. Bacteriophage T3-induced S-adenosylmethionine cleavage.

Authors:  M Gefter; R Hausmann; M Gold; J Hurwitz
Journal:  J Biol Chem       Date:  1966-05-10       Impact factor: 5.157
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  35 in total

1.  Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus: substrate specificity and primary structure analysis.

Authors:  Giovanna Cacciapuoti; Costanzo Bertoldo; Assunta Brio; Vincenzo Zappia; Marina Porcelli
Journal:  Extremophiles       Date:  2003-01-10       Impact factor: 2.395

Review 2.  Determinants of nutrient limitation in cancer.

Authors:  Mark R Sullivan; Matthew G Vander Heiden
Journal:  Crit Rev Biochem Mol Biol       Date:  2019-06-04       Impact factor: 8.250

3.  Regional pattern and heat-resistance of brain 5'-deoxy-5'-methylthioadenosine phosphorylase.

Authors:  A Abbruzzese; G Della Pietra; R Porta
Journal:  Experientia       Date:  1986-07-15

4.  Studies on metabolic role of 5'-Methylthioadenosine in Ochromonas malhamensis and other microorganisms.

Authors:  Y Sugimoto; T Toraya; S Fukui
Journal:  Arch Microbiol       Date:  1976-06       Impact factor: 2.552

5.  Utilization by Saccharomyces cerevisiae of 5'-methylthioadenosine as a source of both purine and methionine.

Authors:  M C Cone; K Marchitto; B Zehfus; A J Ferro
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

6.  Assignment of the gene for methylthioadenosine phosphorylase to human chromosome 9 by mouse-human somatic cell hybridization.

Authors:  C J Carrera; R L Eddy; T B Shows; D A Carson
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

7.  The decarboxylation of S-adenosylmethionine by detergent-treated extracts of rat liver.

Authors:  J Wilson; A Corti; M Hawkins; H G Williams-Ashman; A E Pegg
Journal:  Biochem J       Date:  1979-06-15       Impact factor: 3.857

8.  5'-Alkyl-substituted analogs of 5'-methylthioadenosine as trypanocides.

Authors:  C J Bacchi; J R Sufrin; H C Nathan; A J Spiess; T Hannan; J Garofalo; K Alecia; L Katz; N Yarlett
Journal:  Antimicrob Agents Chemother       Date:  1991-07       Impact factor: 5.191

9.  Synergistic activity of 5-trifluoromethylthioribose and inhibitors of methionine synthesis against Klebsiella pneumoniae.

Authors:  P A Tower; L L Johnson; A J Ferro; J H Fitchen; M K Riscoe
Journal:  Antimicrob Agents Chemother       Date:  1991-08       Impact factor: 5.191

10.  Mild folate deficiency induces genetic and epigenetic instability and phenotype changes in prostate cancer cells.

Authors:  Gaia Bistulfi; Erika Vandette; Sei-Ichi Matsui; Dominic J Smiraglia
Journal:  BMC Biol       Date:  2010-01-21       Impact factor: 7.431
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