| Literature DB >> 535916 |
M S Parshley, J M Cerreta, I Mandl, J A Fierer, G M Turino.
Abstract
A strain of endothelial cells derived from a single cell cloned from a line of normal adult rat lung parenchyma has been maintained in tissue culture for more than 3 years. These cells have been identified as endothelial cells based on the combination of their growth characteristics, cell morphology as observed with both light and electron microscopy, and their physiological properties. They have continued to produce granules, which stain specifically for glycosaminoglycans with Alcian blue, for over 2 1/2 years. During the same period of time, glycosaminoglycans were identified biochemically in both cells and medium. They have maintained the ability to degrade bradykinin over this period as well.Entities:
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Year: 1979 PMID： 535916 DOI： 10.1007/BF02618251
Source DB: PubMed Journal: In Vitro ISSN： 0073-5655