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Literature DB >> 19866772

LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme-Its Localization and Partial Characterization.

K E Kuettner¹, R Eisenstein, L W Soble, C Arsenis.

Abstract

THE LOCALIZATION OF CHICK EMBRYONIC LYSOZYME WAS DETERMINED BY TWO TECHNIQUES: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.

Entities: Species

Year: 1971 PMID： 19866772 PMCID： PMC2108335 DOI： 10.1083/jcb.49.2.450

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

26 in total

LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme-Its Localization and Partial Characterization.

1. On the identity of human lysozymes isolated from normal and abnormal tissues or secretions.

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

3. The significance of the histochemical reaction for carboxyl groups of protein in cartilage matrix.

4. The localization of chloride in hyaline cartilage by histochemical technique.

5. Method for the determination of hexosamines in tissues.

6. The influence of lysozyme on the appearance of epiphyseal cartilage in organ culture.

7. Lysozyme in preosseous cartilage.

8. Lysozyme in embryonic cartilage: ontogenic studies.

9. RAT-KIDNEY LYSOSOMES: ISOLATION AND PROPERTIES.

10. Lysozyme in epiphyseal cartilage. II. The effect of egg white lysozyme on mouse embryonic femurs in organ cultures.

1. Resolution, specificity and transphosphorylase activity of calcifying cartilage alkaline phosphatases.

2. Microchemical studies of acid glycosaminoglycans from isolated chondrocytes in suspension.

3. Microchemical studies on acid glycosaminoglycans of the epiphyseal zones during endochondral calcification.

4. Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells.

5. Immunohistochemical localization of alkaline phosphatase in the chicken epiphyseal growth cartilage.

6. [Concentration of lysozyme during mineralization in callous tissue of healing fractures (author's transl)].

7. Effect of phospholipids on the transformation of amorphous calcium phosphate to hydroxapatite in vitro.

8. Protein and lysozyme content of adult human nucleus pulposus.