Literature DB >> 530273

A simple and fast electrophoretic method for elution of nucleic acids from gels.

Y Chouikh, M Volovitch, P Yot.   

Abstract

A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here.

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Year:  1979        PMID： 530273     DOI： 10.1007/BF00782896

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  13 in total

1.  The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.

Authors:  K N Subramanian; J Pan; S Zain; S M Weissman
Journal:  Nucleic Acids Res       Date:  1974-06       Impact factor: 16.971

2.  A freeze-squeeze method for recovering long DNA from agarose gels.

Authors:  R W Thuring; J P Sanders; P Borst
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

3.  Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes.

Authors:  N Blin; A von Gabain; H Bujard
Journal:  FEBS Lett       Date:  1975-04-15       Impact factor: 4.124

4.  Agarose slab-gel electrophoresis equipment.

Authors:  B Sugden; B De Troy; R J Roberts; J Sambrook
Journal:  Anal Biochem       Date:  1975-09       Impact factor: 3.365

5.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

6.  A rapid method for extracting DNA from agarose gels.

Authors:  M Finkelstein; T H Rownd
Journal:  Plasmid       Date:  1978-09       Impact factor: 3.466

7.  A reliable technique for large-scale DNA separation.

Authors:  A Y Strongin; Y I Kozlov; V G Debabov; R A Arsatians; M L Zlochevsky
Journal:  Anal Biochem       Date:  1977-05-01       Impact factor: 3.365

8.  Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.

Authors:  B Allet; P G Jeppesen; K J Katagiri; H Delius
Journal:  Nature       Date:  1973-01-12       Impact factor: 49.962

9.  Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.

Authors:  K J Danna; G H Sack; D Nathans
Journal:  J Mol Biol       Date:  1973-08-05       Impact factor: 5.469

10.  A restriction map of cauliflower mosaic virus DNA (strain PV 147). Mapping of the cleavage sites of HhaI, SacI, AvaI, PvuII, PstI, XbaI, EcoRI, Bg/II, HincII, HpaII and HindII + III.

Authors:  M Volovitch; G Drugeon; J P Dumas; A L Haenni; P Yot
Journal:  Eur J Biochem       Date:  1979-10
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  10 in total

1.  Transcriptional activation of 2 classes of genes during the hypersensitive reaction of tobacco leaves infiltrated with an incompatible isolate of the phytopathogenic bacterium Pseudomonas solanacearum.

Authors:  Y J Marco; F Ragueh; L Godiard; D Froissard
Journal:  Plant Mol Biol       Date:  1990-07       Impact factor: 4.076

2.  Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis.

Authors:  N Gunge; K Murata; K Sakaguchi
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

3.  Alternative excision products originating from a single integration of polyomavirus DNA.

Authors:  D Huberdeau; B S Sylla; E Herring-Gillam; D Bourgaux-Ramoisy; P Bourgaux
Journal:  Mol Cell Biol       Date:  1985-10       Impact factor: 4.272

4.  Comparative genomic and transcriptomic analyses of trans-kingdom pathogen Fusarium solani species complex reveal degrees of compartmentalization.

Authors:  Daphne Z Hoh; Hsin-Han Lee; Naohisa Wada; Wei-An Liu; Min R Lu; Cheng-Kuo Lai; Huei-Mien Ke; Pei-Feng Sun; Sen-Lin Tang; Wen-Hsin Chung; Ying-Lien Chen; Chia-Lin Chung; Isheng Jason Tsai
Journal:  BMC Biol       Date:  2022-10-20       Impact factor: 7.364

5.  Dimethyl sulfoxide affects the amount of extrachromosomal spleen focus-forming virus DNA in murine erythroleukemia cells.

Authors:  F G Kern; D E Axelrod
Journal:  J Virol       Date:  1983-04       Impact factor: 5.103

6.  Large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes.

Authors:  R K Prakash; R A Schilperoort; M P Nuti
Journal:  J Bacteriol       Date:  1981-03       Impact factor: 3.490

7.  Reduced maturation of Friend virus in adhesive mutants of Friend leukemia cells.

Authors:  C Amici; C Oppi; G Fiorucci; A Battistini; W Djaczenko; G Elia; G B Rossi; A Benedetto
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

8.  Postreplication repair in E. coli: strand exchange reactions of gapped DNA by RecA protein.

Authors:  S C West; E Cassuto; P Howard-Flanders
Journal:  Mol Gen Genet       Date:  1982

9.  Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities.

Authors:  K N Rand; M J Gait
Journal:  EMBO J       Date:  1984-02       Impact factor: 11.598

10.  Can recA protein promote homologous pairing between duplex regions of DNA?

Authors:  E Cassuto; S C West; P Howard-Flanders
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
  10 in total

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