| Literature DB >> 10793753 |
K N Subramanian, J Pan, S Zain, S M Weissman.
Abstract
SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.Mesh:
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Year: 1974 PMID: 10793753 PMCID: PMC343376 DOI: 10.1093/nar/1.6.727
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971