| Literature DB >> 5289031 |
Abstract
Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.Entities:
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Year: 1970 PMID: 5289031 PMCID: PMC283297 DOI: 10.1073/pnas.67.2.951
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205