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Literature DB >> 528231

Acridine orange staining of the mammalian fibroblast cell coat.

Abstract

Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compounds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric--in vitro--measurements and enzymatic digestions, respectively.

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Year: 1979 PMID： 528231 DOI： 10.1007/BF00490098

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

19 in total

1. Heparin and heparin-like substances of cells.

Authors: J E Silbert; H K Kleinman; C K Silbert
Journal: Adv Exp Med Biol Date: 1975 Impact factor: 2.622

Review 2. The morphology of the polysaccharide coat of mammalian cells.

Authors: D F Parsons; J R Subjeck
Journal: Biochim Biophys Acta Date: 1972-02-14

Review 3. Membrane pathology of normal and malignant cells--a review.

Authors: R E Scott; L T Furcht
Journal: Hum Pathol Date: 1976-09 Impact factor: 3.466

4. Morphology and cellular origins of substrate-attached material from mouse fibroblasts.

Authors: J J Rosen; L A Culp
Journal: Exp Cell Res Date: 1977-06 Impact factor: 3.905

5. The nature of substrate-attached materials in human fibroblast cultures: localization of cell and fetal calf serum components.

Authors: C A Heckman; L Vroman; A Pitlick
Journal: Tissue Cell Date: 1977 Impact factor: 2.466

6. In vitro carcinogenesis studies on mouse fibroblast cells.

Authors: K Lapis; G Ferencz; B Szende; I Kovalszky; A Jeney
Journal: Acta Biol Acad Sci Hung Date: 1977

7. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes.

Authors: G L Nicolson; S J Singer
Journal: Proc Natl Acad Sci U S A Date: 1971-05 Impact factor: 11.205

8. Polyacrylamide films as a tool for investigating qualitative and quanitative aspects of the staining of glycosaminoglycans with basic dyes.

Authors: J Tas
Journal: Histochem J Date: 1977-05

9. Substrate-attached glycoproteins mediating adhesion of normal and virus-transformed mouse fibroblasts.

Authors: L A Culp
Journal: J Cell Biol Date: 1974-10 Impact factor: 10.539

10. Relationship of transformation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan.

Authors: R H Cohn; J J Cassiman; M R Bernfield
Journal: J Cell Biol Date: 1976-10 Impact factor: 10.539

6 in total

1. Use of hoechst dyes 33258 and 33342 for enumeration of attached and planktonic bacteria.

Authors: J H Paul
Journal: Appl Environ Microbiol Date: 1982-04 Impact factor: 4.792

Review 2. Cell and tissue fixation, 1972-1982.

Authors: D Hopwood
Journal: Histochem J Date: 1985-04

3. Comparative study on Lewis lung tumor lines with 'low' and 'high' metastatic capacity. II. Cytochemical and biochemical evidence for differences in glycosaminoglycans.

Authors: J Timár; E Móczar; F Timár; K Pál; L Kopper; K Lapis; A Jeney
Journal: Clin Exp Metastasis Date: 1987 Jan-Mar Impact factor: 5.150

4. Flow cytometric measurements and electron microscopy of cell surface glycosaminoglycans using acridine orange.

Authors: J Timar; F Boldog; L Kopper; K Lapis
Journal: Histochem J Date: 1985-01

5. The use of fluorochromes in the cytochemical characterization of some phytoflagellates.

Authors: M E Klut; T Bisalputra; N J Antia
Journal: Histochem J Date: 1988-01

6. Acridine orange stabilization of glycosaminoglycans in beginning endochondral ossification. A comparative light and electron microscopic study.

Authors: N Shepard; N Mitchell
Journal: Histochemistry Date: 1981

6 in total