Literature DB >> 528061

Simple and rapid procedure for the selective removal of lysozyme from human saliva.

G R Germaine, L M Tellefson.   

Abstract

A simple and rapid method is described for the removal of lysozyme from human whole salivary supernatant. Saliva specimens were adsorbed with Micrococcus lysodeikticus. The saliva so treated was depleted of 95% of the lysozyme activity. Changes in total protein, lactoperoxidase, lactoferrin, immunoglobulin A, and the proportions of several anionic proteins were less than 10%. It is concluded that adsorption of saliva with M. lysodeikticus is a suitable procedure for the preparation of saliva that is selectively deficient in lysozyme.

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Year:  1979        PMID： 528061      PMCID： PMC414716          DOI： 10.1128/iai.26.3.991-995.1979

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  18 in total

1.  Studies on the Antibacterial Factors of Human Saliva.

Authors:  M Van Kesteren; B G Bibby; G P Berry
Journal:  J Bacteriol       Date:  1942-05       Impact factor: 3.490

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme.

Authors:  D SHUGAR
Journal:  Biochim Biophys Acta       Date:  1952-03

4.  The in-vitro attachment of an oral Streptococcus sp. to the acquired tooth enamel pellicle.

Authors:  D Orstavik
Journal:  Arch Oral Biol       Date:  1978       Impact factor: 2.633

5.  A bactericidal effect for human lactoferrin.

Authors:  R R Arnold; M F Cole; J R McGhee
Journal:  Science       Date:  1977-07-15       Impact factor: 47.728

6.  Human secretory immunoglobulins. I. Salivary secretions from individuals with normal or low levels of serum immunoglobulins.

Authors:  P Brandtzaeg; I Fjellanger; S T Gjeruldsen
Journal:  Scand J Haematol Suppl       Date:  1970

Review 7.  Secretory immunoglobulins.

Authors:  T B Tomasi; J Bienenstock
Journal:  Adv Immunol       Date:  1968       Impact factor: 3.543

8.  Secretory immunity and immunodeficiency.

Authors:  R R Arnold; S J Prince; J Mestecky; D Lynch; M Lynch; J R McGhee
Journal:  Adv Exp Med Biol       Date:  1978       Impact factor: 2.622

9.  Proteolytic activity of Candida albicans: action on human salivary proteins.

Authors:  G R Germaine; L M Tellefson; G L Johnson
Journal:  Infect Immun       Date:  1978-12       Impact factor: 3.441

10.  Alterations in lactoferrin in salivary gland disease.

Authors:  L Tabak; I D Mandel; D Karlan; H Baurmash
Journal:  J Dent Res       Date:  1978-01       Impact factor: 6.116
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  8 in total

1.  Increases in salivary lysozyme and IgA concentrations and secretory rates independent of salivary flow rates following viewing of humorous videotape.

Authors:  S Perera; E Sabin; P Nelson; D Lowe
Journal:  Int J Behav Med       Date:  1998

2.  Salivary lysozyme: a noninvasive marker for the study of the effects of stress of natural immunity.

Authors:  S Perera; M Uddin; J A Hayes
Journal:  Int J Behav Med       Date:  1997

3.  Potential role of lysozyme in bactericidal activity of in vitro-acquired salivary pellicle against Streptococcus faecium 9790.

Authors:  G R Germaine; L M Tellefson
Journal:  Infect Immun       Date:  1986-12       Impact factor: 3.441

4.  Effect of lysozyme on glucose fermentation, cytoplasmic pH, and intracellular potassium concentrations in Streptococcus mutans 10449.

Authors:  Y B Wang; G R Germaine
Journal:  Infect Immun       Date:  1991-02       Impact factor: 3.441

5.  Role of the lysozyme inhibitor Ivy in growth or survival of Escherichia coli and Pseudomonas aeruginosa bacteria in hen egg white and in human saliva and breast milk.

Authors:  Daphne Deckers; Dietrich Vanlint; Lien Callewaert; Abram Aertsen; Chris W Michiels
Journal:  Appl Environ Microbiol       Date:  2008-05-30       Impact factor: 4.792

6.  Effect of human saliva on glucose uptake by Streptococcus mutans and other oral microorganisms.

Authors:  G R Germaine; L M Tellefson
Journal:  Infect Immun       Date:  1981-02       Impact factor: 3.441

7.  Adsorption of lysozyme from human whole saliva by Streptococcus sanguis 903 and other oral microorganisms.

Authors:  N J Laible; G R Germaine
Journal:  Infect Immun       Date:  1982-04       Impact factor: 3.441

8.  Relationships between levels of lysozyme, lactoferrin, salivary peroxidase, and secretory immunoglobulin A in stimulated parotid saliva.

Authors:  J D Rudney; Q T Smith
Journal:  Infect Immun       Date:  1985-09       Impact factor: 3.441
  8 in total

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