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Literature DB >> 521457

Ultrastructure of double minutes from a human tumor cell line.

Abstract

Double minutes (dm) have been isolated from human tumor cells by zonal centrifugation and by differential pelleting of chromosome suspsension. These methods allowed collection of dm in sufficient quantity and purity for visualization with electron microscopy. Ultrastructurally, the chromatin fibers in dm resemble thrance fragments was found. When the two isolation protocols were compared, differential pelleting was shown to increase purity twofold to 85% dm by mass. The differential pelleting procedure enables easy collection of dm in sufficient quantity and purity for chemical analysis.

Entities: Disease Species

Mesh：

Substances：
Chromatin

Year: 1979 PMID： 521457 PMCID： PMC2110517 DOI： 10.1083/jcb.83.3.663

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

15 in total

1. MINUTE CHROMATIN BODIES IN MALIGNANT TUMOURS OF CHILDHOOD.

Authors: D COX; C YUNCKEN; A I SPRIGGS
Journal: Lancet Date: 1965-07-10 Impact factor: 79.321

2. Experimental elimination and recovery of double minute chromosomes in malignant cell populations.

Authors: G Levan; N Mandahl; B O Bengtsson; A Levan
Journal: Hereditas Date: 1977 Impact factor: 3.271

3. ICN-UCLA Symposia. Biological recognition and assembly. March 4 - March 9, 1979. Abstracts.

Authors:
Journal: J Supramol Struct Suppl Date: 1979

4. Are double minutes chromosomes?

Authors: P E Barker; T C Hsu
Journal: Exp Cell Res Date: 1978-05 Impact factor: 3.905

5. Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

Authors: F W Alt; R E Kellems; J R Bertino; R T Schimke
Journal: J Biol Chem Date: 1978-03-10 Impact factor: 5.157

6. Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line.

Authors: G Balaban-Malenbaum; F Gilbert
Journal: Science Date: 1977-11-18 Impact factor: 47.728

7. A new chromosome type replacing the double minutes in a mouse tumor.

Authors: A Levan; G Levan; N Mandahl
Journal: Cytogenet Cell Genet Date: 1978

8. Specific measurement of DNA in nuclei and nucleic acids using diaminobenzoic acid.

Authors: P S Thomas; M N Farquhar
Journal: Anal Biochem Date: 1978-08-15 Impact factor: 3.365

9. Double minutes in human carcinoma cell lines, with special reference to breast tumors.

Authors: P E Barker; T C Hsu
Journal: J Natl Cancer Inst Date: 1979-02 Impact factor: 13.506

10. Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV.

Authors: T G Padgett; E Stubbledield; H E Varmus
Journal: Cell Date: 1977-04 Impact factor: 41.582

10 in total

1. Analysis of the replication mode of double minutes using the PCC technique combined with BrdUrd labeling.

Authors: S Takayama; Y Uwaike
Journal: Chromosoma Date: 1988-11 Impact factor: 4.316

2. Ultrastructural features of minute chromosomes in a methotrexate-resistant mouse 3T3 cell line.

Authors: B A Hamkalo; P J Farnham; R Johnston; R T Schimke
Journal: Proc Natl Acad Sci U S A Date: 1985-02 Impact factor: 11.205

3. Deoxyribonucleic acid-mediated gene transfer in mammalian cells: molecular analysis of unstable transformants and their progression to stability.

Authors: G A Scangos; K M Huttner; D K Juricek; F H Ruddle
Journal: Mol Cell Biol Date: 1981-02 Impact factor: 4.272

4. Occurrence and evolution of homogeneously staining regions may be due to breakage-fusion-bridge cycles following telomere loss.

Authors: J K Cowell; O J Miller
Journal: Chromosoma Date: 1983 Impact factor: 4.316

5. General method for cloning amplified DNA by differential screening with genomic probes.

Authors: O Brison; F Ardeshir; G R Stark
Journal: Mol Cell Biol Date: 1982-05 Impact factor: 4.272

6. Double minute chromosomes carrying the human multidrug resistance 1 and 2 genes are generated from the dimerization of submicroscopic circular DNAs in colchicine-selected KB carcinoma cells.

Authors: P V Schoenlein; D W Shen; J T Barrett; I Pastan; M M Gottesman
Journal: Mol Biol Cell Date: 1992-05 Impact factor: 4.138

7. Co-amplification of double minute chromosomes, multiple drug resistance, and cell surface P-glycoprotein in DNA-mediated transformants of mouse cells.

Authors: S M Robertson; V Ling; C P Stanners
Journal: Mol Cell Biol Date: 1984-03 Impact factor: 4.272

8. Relationship of amplified dihydrofolate reductase genes to double minute chromosomes in unstably resistant mouse fibroblast cell lines.

Authors: P C Brown; S M Beverley; R T Schimke
Journal: Mol Cell Biol Date: 1981-12 Impact factor: 4.272

9. Regular pattern of karyotypic alterations accompanying gene amplification in Djungarian hamster cells: study of colchicine, adriablastin, and methotrexate resistance.

Authors: B P Kopnin; J S Massino; A V Gudkov
Journal: Chromosoma Date: 1985 Impact factor: 4.316

10. Localization of a bidirectional DNA replication origin in the native locus and in episomally amplified murine adenosine deaminase loci.

Authors: S M Carroll; M L DeRose; J L Kolman; G H Nonet; R E Kelly; G M Wahl
Journal: Mol Cell Biol Date: 1993-05 Impact factor: 4.272

10 in total