Literature DB >> 5158915

Digestion of an exogenous protein by rat yolk-sac cultured in vitro.

K E Williams, J B Lloyd, M Davies, F Beck.   

Abstract

Yolk-sacs were removed from 17.5-day pregnant rats injected 2-5h previously with (125)I-labelled bovine serum albumin. The specific activities of acid phosphatase and acid proteinase, and the specific radioactivities (trichloroacetic acid-insoluble and trichloroacetic acid-soluble) were measured in subcellular fractions prepared by homogenization and differential centrifugation. The conversion of acid-insoluble into acid-soluble radioactivity within cultured tissue was followed and the nature of the liberated products was investigated by gel chromatography. The results are consistent with the protein entering lysosomes and being digested there. The radiolabel was released chiefly as free iodotyrosine.

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Year:  1971        PMID： 5158915      PMCID： PMC1178054          DOI： 10.1042/bj1250303

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Studies on the mechanism of teratogenic action of trypan blue.

Authors:  J G WILSON; A R BEAUDOIN; H J FREE
Journal:  Anat Rec       Date:  1959-02

2.  SEPARATION OF SOME IODOAMINO ACIDS AND IODIDE BY GEL FILTRATION.

Authors:  E H MOUGEY; J W MASON
Journal:  Anal Biochem       Date:  1963-09       Impact factor: 3.365

3.  SEPARATION OF AMINO ACIDS, AS COPPER CHELATES, FROM AMINO ACID, PROTEIN AND PEPTIDE MIXTURES.

Authors:  S FAZAKERLEY; D R BEST
Journal:  Anal Biochem       Date:  1965-08       Impact factor: 3.365

4.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors:  C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal:  Biochem J       Date:  1955-08       Impact factor: 3.857

5.  Effects of ovariectomy and hypervitaminosis A on lysosomes of the rat conceptus.

Authors:  R L Schultz
Journal:  Teratology       Date:  1969-11

6.  Studies on acid hydrolases in adult and foetal tissues. Acid rho-nitrophenyl phosphate phosphohydrolases of adult guinea-pig liver.

Authors:  M W Neil; M W Horner
Journal:  Biochem J       Date:  1964-07       Impact factor: 3.857

7.  The digestive capacity of rat yolk-sac in organ culture.

Authors:  K E Williams; J B Lloyd; F Beck
Journal:  Biochem J       Date:  1969-12       Impact factor: 3.857

8.  [Experimental studies on the yolk sac epithelium of the rat].

Authors:  S Krzyzowska-Gruca; T H Schiebler
Journal:  Z Zellforsch Mikrosk Anat       Date:  1967

9.  The uptake and digestion of iodinated human serum albumin by macrophages in vitro.

Authors:  B A Ehrenreich; Z A Cohn
Journal:  J Exp Med       Date:  1967-11-01       Impact factor: 14.307

10.  Fate of hemoglobin pincytosed by macrophages in vitro.

Authors:  B A Ehrenreich; Z A Cohn
Journal:  J Cell Biol       Date:  1968-07       Impact factor: 10.539
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  11 in total

1.  A kinetic analysis of pinocytosis in rat yolk-sac.

Authors:  J B Lloyd; K E Williams; F Beck; M E Kidston
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

2.  Hydrolysis of an exogenous 125I-labelled protein by rat yolk sacs. Evidence for intracellular degradation within lysosomes.

Authors:  G Livesey; K E Williams
Journal:  Biochem J       Date:  1979-12-15       Impact factor: 3.857

3.  Rates of pinocytic capture of simple proteins by rat yolk sacs incubated in vitro.

Authors:  G Livesey; K E Williams
Journal:  Biochem J       Date:  1981-09-15       Impact factor: 3.857

4.  Rate of pinocytic capture of macromolecular substrates by rat yolk sac incubated in serum-free culture medium.

Authors:  G E Ibbotson; K E Williams
Journal:  Biochem J       Date:  1979-03-15       Impact factor: 3.857

5.  A quantitative study of pinocytosis and intracellular proteolysis in rat peritoneal macrophages.

Authors:  M K Pratten; K E Williams; J B Lloyd
Journal:  Biochem J       Date:  1977-12-15       Impact factor: 3.857

6.  The effect of chemical treatments of albumin and orosomucoid on rate of clearance from the rat bloodstream and rate of pinocytic capture of rat yolk sac cultured in vitro.

Authors:  A T Moore; K E Williams; J B Lloyd
Journal:  Biochem J       Date:  1977-06-15       Impact factor: 3.857

7.  Comparative placental morphology and function.

Authors:  F Beck
Journal:  Environ Health Perspect       Date:  1976-12       Impact factor: 9.031

8.  Degradation of proteins microinjected into IMR-90 human diploid fibroblasts.

Authors:  N T Neff; L Bourret; P Miao; J F Dice
Journal:  J Cell Biol       Date:  1981-10       Impact factor: 10.539

9.  Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro.

Authors:  K E Williams; E M Kidston; F Beck; J B Lloyd
Journal:  J Cell Biol       Date:  1975-01       Impact factor: 10.539

10.  Protein absorption by renal cells. II. Very rapid lysosomal digestion of exogenous ribonuclease in vitro.

Authors:  S J Davidson
Journal:  J Cell Biol       Date:  1973-10       Impact factor: 10.539
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