| Literature DB >> 5158502 |
A Spector, L K Li, R C Augusteyn, A Schneider, T Freund.
Abstract
alpha-Crystallin was isolated from calf lens periphery by chromatography on DEAE-cellulose and gel filtration. Three distinct populations of macromolecules have been isolated with molecular weights in the ranges approx. 6x10(5)-9x10(5), 0.9x10(6)-4x10(6) and greater than 10x10(6). The concentration of macromolecules at the molecular-weight limits of a population are very low. The members of the different populations do not appear to be in equilibrium with each other. Further, in those molecular-weight fractions investigated, no equilibrium between members of the same population was observed. The population of lowest molecular weight comprises 65-75% of the total material. The amino acid and subunit composition of the different-sized fractions appear very similar, if not identical. The only chemical difference observed between the fractions is the presence of significant amounts of sugar in the higher-molecular-weight fractions. Subunit molecular weights of approx. 19.5x10(3) and 22.5x10(3) were observed for all alpha-crystallin fractions.Entities:
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Year: 1971 PMID： 5158502 PMCID： PMC1177148 DOI： 10.1042/bj1240337
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857