Literature DB >> 5118103

Effect of nitrogenase components from mutant and wild-type strains of Azotobacter on the dilution effect of nitrogenase.

G J Sorger.   

Abstract

Nitrogenase activity exhibits a dilution effect. Evidence is presented that the reason for the dilution effect is that one of the component proteins of nitrogenase is limiting in preparations of this enzyme. The limiting component appears to be the non-haem-iron-containing protein (also called fraction II, iron protein, azoferredoxin), which is equivalent to the enhancement factor for nitrogenase activity present in extracts of nitrogenaseless mutant 22R1. A mathematical function of specific activity is described that is useful in describing nitrogenase. It takes into account the dilution effect and the exponential nature of the relationship between nitrogenase activity and enzyme protein concentration.

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Year:  1971        PMID： 5118103      PMCID： PMC1176779          DOI： 10.1042/bj1220305

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  Effect of non-haem iron proteins and cytochrome C from Azotobacter upon the activity and oxygen sensitivity of Azobacter nitrogenase.

Authors:  M G. Yates
Journal:  FEBS Lett       Date:  1970-06-27       Impact factor: 4.124

2.  FERREDOXIN AND ATP, REQUIREMENTS FOR NITROGEN FIXATION IN CELL-FREE EXTRACTS OF CLOSTRIDIUM PASTEURIANUM.

Authors:  L E MORTENSON
Journal:  Proc Natl Acad Sci U S A       Date:  1964-08       Impact factor: 11.205

3.  N,O-Diacetylneuraminic acid and N-acetylneuraminic acid in Escherichia coli.

Authors:  C W DE WITT; J A ROWE
Journal:  Nature       Date:  1959-08-01       Impact factor: 49.962

4.  The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.

Authors:  W A Bulen; J R LeComte
Journal:  Proc Natl Acad Sci U S A       Date:  1966-09       Impact factor: 11.205

5.  Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum.

Authors:  M J Dilworth
Journal:  Biochim Biophys Acta       Date:  1966-10-31

6.  Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules.

Authors:  R V Klucas; B Koch; S A Russell; H J Evans
Journal:  Plant Physiol       Date:  1968-12       Impact factor: 8.340

7.  The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin.

Authors:  D C Yoch; J R Benemann; R C Valentine; D I Arnon
Journal:  Proc Natl Acad Sci U S A       Date:  1969-12       Impact factor: 11.205

8.  Regulation of nitrogen fixation in Azotobacter vinelandii OP: the role of nitrate reductase.

Authors:  G J Sorger
Journal:  J Bacteriol       Date:  1969-04       Impact factor: 3.490

9.  The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.

Authors:  J R Benemann; D C Yoch; R C Valentine; D I Arnon
Journal:  Proc Natl Acad Sci U S A       Date:  1969-11       Impact factor: 11.205

10.  Reduction of N2 by complementary functioning of two components from nitrogen-fixing bacteria.

Authors:  R W Detroy; D F Witz; R A Parejko; P W Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1968-10       Impact factor: 11.205
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  2 in total

1.  Bacterial diversity of East Calcutta Wet land area: possible identification of potential bacterial population for different biotechnological uses.

Authors:  Abhrajyoti Ghosh; Bhaswar Maity; Krishanu Chakrabarti; Dhrubajyoti Chattopadhyay
Journal:  Microb Ecol       Date:  2007-05-20       Impact factor: 4.552

2.  Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins.

Authors:  R R Eady; B E Smith; K A Cook; J R Postgate
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857
  2 in total

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