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Literature DB >> 16656989

Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules.

R V Klucas¹, B Koch, S A Russell, H J Evans.

Abstract

The nitrogenase system in cell-free extracts of soybean nodule bacteroids was fractionated into 2 components by use of protamine sulfate or polypropylene glycol precipitation followed by chromatography on DEAE-cellulose. Iron and molybdenum were concentrated in 1 fraction and iron in the other. Combination of fractions resulted in a striking stimulation in activity relative to the activity of individual fractions. The effect of different proportions of the 2 fractions on specific activities was studied. The ratios of the rates of reduction of acetylene and N(2) by extracts or fractions of different purities were relatively constant. Extracts or fractions retained most of their nitrogenase activities when stored in liquid N(2).

Entities: Chemical Species

Year: 1968 PMID： 16656989 PMCID： PMC1087102 DOI： 10.1104/pp.43.12.1906

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

14 in total

1. NITROGEN FIXATION: HYDROSULFITE AS ELECTRON DONOR WITH CELL-FREE PREPARATIONS OF AZOTOBACTER VINELANDII AND RHODOSPIRILLUM RUBRUM.

Authors: W A BULEN; R C BURNS; J R LECOMTE
Journal: Proc Natl Acad Sci U S A Date: 1965-03 Impact factor: 11.205

2. Purification, metal composition and properties of molybdoferredoxin and azoferredoxin, two of the components of the nitrogen-fixing system of Clostridium pasteurianum.

Authors: L E Mortenson; J A Morris; D Y Jeng
Journal: Biochim Biophys Acta Date: 1967-08-29

3. Method for demonstrating cofactor requirements for nitrogen fixation.

Authors: T O Munson; M J Dilworth; R H Burris
Journal: Biochim Biophys Acta Date: 1965-06-15

4. The adenosine triphosphate requirement for nitrogen fixation in cell-free extracts of Clostridium pasteurianum.

Authors: M J Dilworth; D Subramanian; T O Munson; R H Burris
Journal: Biochim Biophys Acta Date: 1965-06-22

5. The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.

Authors: W A Bulen; J R LeComte
Journal: Proc Natl Acad Sci U S A Date: 1966-09 Impact factor: 11.205

6. Reduction of azide by the N2-fixing enzyme system.

Authors: R Schöllhorn; R H Burris
Journal: Proc Natl Acad Sci U S A Date: 1967-05 Impact factor: 11.205

7. Properties of the nitrogenase system in cell-free extracts of bacteroids from soybean root nodules.

Authors: B Koch; H J Evans; S Russell
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

8. Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules.

Authors: J F Bergersen; G L Turner
Journal: Biochim Biophys Acta Date: 1967-08-29

9. Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum.

Authors: M J Dilworth
Journal: Biochim Biophys Acta Date: 1966-10-31

10. Some properties of nitrogen-fixing breis prepared from soybean root nodules.

Authors: F J Bergersen
Journal: Biochim Biophys Acta Date: 1966-12-28

12 in total

1. Symbiotic effectiveness and n(2) fixation in nodulated soybean.

Authors: C Sloger
Journal: Plant Physiol Date: 1969-12 Impact factor: 8.340

Review 2. Dinitrogen (N 2 ) fixation (with a biochemical emphasis).

Authors: H Dalton; L E Mortenson
Journal: Bacteriol Rev Date: 1972-06

3. The mechanism of ammonia assimilation in nitrogen fixing Bacteria.

Authors: H Nagatani; M Shimizu; R C Valentine
Journal: Arch Mikrobiol Date: 1971

4. Kinetic studies of nitrogenase from soya-bean root-nodule bacteroids.

Authors: F J Bergersen; G L Turner
Journal: Biochem J Date: 1973-01 Impact factor: 3.857

5. Effect of nitrogenase components from mutant and wild-type strains of Azotobacter on the dilution effect of nitrogenase.

Authors: G J Sorger
Journal: Biochem J Date: 1971-04 Impact factor: 3.857

6. Purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (Glycine max Merr) nodules.

Authors: B Koch; P Wong; S A Russell; R Howard; H J Evans
Journal: Biochem J Date: 1970-08 Impact factor: 3.857

7. Complementary functioning of the component proteins of nitrogenase from several bacteria.

Authors: D W Emerich; R H Burris
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

8. Poly-beta-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase Activity.

Authors: P P Wong; H J Evans
Journal: Plant Physiol Date: 1971-06 Impact factor: 8.340

9. Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes.

Authors: A Nason; K Y Lee; S S Pan; P A Ketchum; A Lamberti; J DeVries
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

10. Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

Authors: D W Emerich; T Ruiz-Argüeso; T M Ching; H J Evans
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490