Literature DB >> 5114970

Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography.

R Fields, H B Dixon, G R Law, C Yui.   

Abstract

A procedure is described for isolating the enzyme ribonuclease T(1) from Takadiastase, an extract of the mould Aspergillus oryzae. It involves an initial concentration of the enzyme by adsorption on DEAE-cellulose followed by gradient elution. Later the enzyme is chromatographed on the same adsorbent with an eluent of constant composition. Yields of 350-380mg of ribonuclease T(1) from 500g of Takadiastase were obtained.

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Year:  1971        PMID： 5114970      PMCID： PMC1176633          DOI： 10.1042/bj1210591

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  STRUCTURE OF A RIBONUCLEIC ACID.

Authors:  R W HOLLEY; J APGAR; G A EVERETT; J T MADISON; M MARQUISEE; S H MERRILL; J R PENSWICK; A ZAMIR
Journal:  Science       Date:  1965-03-19       Impact factor: 47.728

3.  A SIMPLIFIED METHOD FOR THE PURIFICATION OF RIBONUCLEASE T.

Authors:  T UCHIDA
Journal:  J Biochem       Date:  1965-04       Impact factor: 3.387

4.  Tertiary structure of ribonuclease.

Authors:  G Kartha; J Bello; D Harker
Journal:  Nature       Date:  1967-03-04       Impact factor: 49.962

Review 5.  Ribonucleases.

Authors:  E A Barnard
Journal:  Annu Rev Biochem       Date:  1969       Impact factor: 23.643

6.  Molecular weight estimation of polypeptides by SDS-polyacrylamide gel electrophoresis: further data concerning resolving power and general considerations.

Authors:  A L Shapiro; J V Maizel
Journal:  Anal Biochem       Date:  1969-06       Impact factor: 3.365

7.  The sequence of 5 s ribosomal ribonucleic acid.

Authors:  G G Brownlee; F Sanger; B G Barrell
Journal:  J Mol Biol       Date:  1968-06-28       Impact factor: 5.469

8.  Studies on nonspecific adenosine deaminase from Takadiastase. I. Purification and properties.

Authors:  S Minato; T Tagawa; K Nakanishi
Journal:  J Biochem       Date:  1965-12       Impact factor: 3.387

9.  The structure of ribonuclease-S at 3.5 A resolution.

Authors:  H W Wyckoff; K D Hardman; N M Allewell; T Inagami; L N Johnson; F M Richards
Journal:  J Biol Chem       Date:  1967-09-10       Impact factor: 5.157

10.  Chromatography of oxidized and reduced cytochrome c on carboxymethylcellulose.

Authors:  H B Dixon; C M Thompson
Journal:  Biochem J       Date:  1968-04       Impact factor: 3.857
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  4 in total

1.  Isolation and characterization of recombinant Drosophila Copia aspartic proteinase.

Authors:  Senarath B P Athauda; Katsuji Yoshioka; Tadayoshi Shiba; Kenji Takahashi
Journal:  Biochem J       Date:  2006-11-01       Impact factor: 3.857

2.  Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.

Authors:  R Fields; H B Dixon
Journal:  Biochem J       Date:  1971-02       Impact factor: 3.857

3.  The measurement of amino groups in proteins and peptides.

Authors:  R Fields
Journal:  Biochem J       Date:  1971-09       Impact factor: 3.857

4.  Kinetics of tryptic hydrolysis of the arginine-valine bond in folded and unfolded ribonuclease T1.

Authors:  C N Pace; A J Barrett
Journal:  Biochem J       Date:  1984-04-15       Impact factor: 3.857
  4 in total

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