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Literature DB >> 5114969

Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.

Abstract

A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine and with transaminated ribonuclease T(1) is presented; the isolation of protein hydrazones is discussed.

Entities: Chemical

Mesh：

Substances：

Year: 1971 PMID： 5114969 PMCID： PMC1176632 DOI： 10.1042/bj1210587

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

7 in total

1. Conversion of the N-terminal serine residue of corticotrophin into glycine.

Authors: H B DIXON; L R WEITKAMP
Journal: Biochem J Date: 1962-09 Impact factor: 3.857

2. Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography.

Authors: R Fields; H B Dixon; G R Law; C Yui
Journal: Biochem J Date: 1971-02 Impact factor: 3.857

3. L-phenylalanine ammonia-lyase. II. Mechanism and kinetic properties of the enzyme from potato tubers.

Authors: E A Havir; K R Hanson
Journal: Biochemistry Date: 1968-05 Impact factor: 3.162

4. Histidine decarboxylase of Lactobacillus 30a. IV. The presence of covalently bound pyruvate as the prosthetic group.

Authors: W D Riley; E E Snell
Journal: Biochemistry Date: 1968-10 Impact factor: 3.162

5. Transamination of the N-terminal residue of carboxypeptidase.

Authors: S van Heyningen; K F Tipton; H B Dixon
Journal: Biochem J Date: 1968-07 Impact factor: 3.857

6. Transamination of peptides.

Authors: H B Dixon
Journal: Biochem J Date: 1964-09 Impact factor: 3.857

7. A spectrophotometric method for the microdetermination of periodate.

Authors: R Fields; H B Dixon
Journal: Biochem J Date: 1968-08 Impact factor: 3.857

7 in total

18 in total

1. An alkyl etherase in rat liver.

Authors: R A Peters; M Shorthouse
Journal: J Physiol Date: 1975-11 Impact factor: 5.182

2. The modification and removal of the N-terminal residue of trypsin by transamination.

Authors: D Webster; R E Offord
Journal: Biochem J Date: 1972-11 Impact factor: 3.857

3. The measurement of amino groups in proteins and peptides.

Authors: R Fields
Journal: Biochem J Date: 1971-09 Impact factor: 3.857

4. Impact of silicon-based quantum dots on the antioxidative system in white muscle of Carassius auratus gibelio.

Authors: Loredana Stanca; Sorina Nicoleta Petrache; Mihaela Radu; Andreea Iren Serban; Maria Cristina Munteanu; Daniela Teodorescu; Andreea Cristina Staicu; Cornelia Sima; Marieta Costache; Constantin Grigoriu; Otilia Zarnescu; Anca Dinischiotu
Journal: Fish Physiol Biochem Date: 2011-12-04 Impact factor: 2.794

Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.

1. Conversion of the N-terminal serine residue of corticotrophin into glycine.

2. Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography.

3. L-phenylalanine ammonia-lyase. II. Mechanism and kinetic properties of the enzyme from potato tubers.

4. Histidine decarboxylase of Lactobacillus 30a. IV. The presence of covalently bound pyruvate as the prosthetic group.

5. Transamination of the N-terminal residue of carboxypeptidase.

6. Transamination of peptides.

7. A spectrophotometric method for the microdetermination of periodate.

1. An alkyl etherase in rat liver.

2. The modification and removal of the N-terminal residue of trypsin by transamination.

3. The measurement of amino groups in proteins and peptides.

4. Impact of silicon-based quantum dots on the antioxidative system in white muscle of Carassius auratus gibelio.

5. Carnosine protects against the neurotoxic effects of a serotonin-derived melanoid.

6. Homeostatic regulation of intracellular hydrogen peroxide concentration in aerobically growing Escherichia coli.

7. Isolation and some properties of glycated D-glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.

8. Reaction of glycolaldehyde with proteins: latent crosslinking potential of alpha-hydroxyaldehydes.

9. Imparting bone affinity to glycoproteins through the conjugation of bisphosphonates.

10. Roles of catalase and glutathione peroxidase in the tolerance of a pulmonate gastropod to anoxia and reoxygenation.