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Literature DB >> 5106347

Transcription of the influenza ribonucleic acid genome by a virion polymerase. II. Nature of the in vitro polymerase product.

Abstract

The properties of the ribonucleic acid (RNA) synthesized by the influenza (WSN) virion polymerase have been investigated. Most of the product RNA is synthesized in association with virion RNA species from which it can be released by heat treatment as single-stranded, ribonuclease-sensitive polynucleotides (average molecular weight, 2-hr sample, about 10(5) daltons). At least 95% of the product is complementary to the viral RNA species. On the basis of the molar ratio of the RNA species isolated from a (3)H-uridine-labeled virus preparation, it was calculated that the WSN genome consists of seven pieces of RNA with a sum molecular weight of about 5 x 10(6) daltons.

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Year: 1971 PMID： 5106347 PMCID： PMC356216

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

18 in total

Transcription of the influenza ribonucleic acid genome by a virion polymerase. II. Nature of the in vitro polymerase product.

1. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion.

2. RNA-dependent DNA polymerase in virions of RNA tumour viruses.

3. RNA-dependent DNA polymerase in virions of Rous sarcoma virus.

4. RNA polymerase activity in purified reoviruses.

5. Polyacrylamide gel electrophoresis of influenza virus RNA.

6. Molecular weights of ribosomal RNA in relation to evolution.

7. Electrophoretic separation of viral nucleic acids on polyacrylamide gels.

8. The RNA of influenza virus.

9. Isolation and properties of poliovirus minus strand ribonucleic acid.

10. Nonpermissive infections of mammalian cells: synthesis of inluenza virus genome in HeLa cells.

1. Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures.

2. Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts.

3. Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis.

4. Influenza virus genome consists of eight distinct RNA species.

5. Transcriptase activity and genome composition of defective influenza virus.

6. Influenza virion RNA-dependent RNA polymerase: stimulation by guanosine and related compounds.

7. Temperature-sensitive virion transcriptase activity in mutants of WSN influenza virus.

8. RNAs of influenza A, B, and C viruses.

9. Structural components of influenza C virions.

10. Influenza virion transcriptase: synthesis in vitro of large, polyadenylic acid-containing complementary RNA.