Literature DB >> 50952

A combination of sister chromatid differential staining and giemsa banding.

S Pathak, A D Stock, A Lusby.   

Abstract

We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists.

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Year:  1975        PMID： 50952     DOI： 10.1007/bf02358850

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  7 in total

1.  Localization of sister chromatid exchanges in human chromosomes.

Authors:  S A Latt
Journal:  Science       Date:  1974-07-05       Impact factor: 47.728

2.  New Giemsa method for the differential staining of sister chromatids.

Authors:  P Perry; S Wolff
Journal:  Nature       Date:  1974-09-13       Impact factor: 49.962

3.  Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

Authors:  S A Latt
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

4.  Banding pattern analysis of human chromosomes by use of a urea treatment technique.

Authors:  Y Shiraishi; T H Yosida
Journal:  Chromosoma       Date:  1972       Impact factor: 4.316

5.  Giemsa technique for the detection of sister chromatid exchanges.

Authors:  J R Korenberg; E F Freedlender
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

6.  A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes.

Authors:  R S Chaganti; S Schonberg; J German
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

7.  Differential Giemsa staining of sister chromatids and the study of chromatid exchanges without autoradiography.

Authors:  S Wolff; P Perry
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316
  7 in total
  21 in total

1.  Sister chromatid exchange points in the heterochromatin and euchromatin regions of Chinese hedgehog chromosomes.

Authors:  Y Yongshan; S Ficin
Journal:  Theor Appl Genet       Date:  1987-01       Impact factor: 5.699

2.  Genetic and molecular analysis of chlorambucil-induced germ-line mutations in the mouse.

Authors:  E M Rinchik; J W Bangham; P R Hunsicker; N L Cacheiro; B S Kwon; I J Jackson; L B Russell
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

3.  Sister chromatid differential staining pattern in prematurely condensed chromosomes.

Authors:  Y F Lau; W N Hittelman; F E Arrighi
Journal:  Experientia       Date:  1976

4.  Rate of sister chromatid exchanges in mammalian cells differing in diploid numbers.

Authors:  S Pathak; O G Ward; T C Hsu
Journal:  Experientia       Date:  1977-07-15

5.  Relation between the SCE points and the DNA replication bands.

Authors:  J J Hoo; M I Parslow
Journal:  Chromosoma       Date:  1979-06-21       Impact factor: 4.316

6.  The frequency and distribution of sister chromatid exchanges in human chromosomes.

Authors:  W F Morgan; P E Crossen
Journal:  Hum Genet       Date:  1977-10-14       Impact factor: 4.132

7.  Molecular and genetic characterization of a radiation-induced structural rearrangement in mouse chromosome 2 causing mutations at the limb deformity and agouti loci.

Authors:  R P Woychik; W M Generoso; L B Russell; K T Cain; N L Cacheiro; S J Bultman; P B Selby; M E Dickinson; B L Hogan; J C Rutledge
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

8.  Considerations on the mechanism of differential Giemsa staining of BrdU-substituted chromosomes.

Authors:  G Speit
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

9.  A high resolution study of the DNA replication patterns of chinese hamster chromosomes using sister chromatid differential staining technique.

Authors:  P E Crossen; S Pathak; F E Arrighi
Journal:  Chromosoma       Date:  1975-11-11       Impact factor: 4.316

10.  Differential rates of sister chromatid exchanges between euchromatin and heterochromatin.

Authors:  T C Hsu; S Pathak
Journal:  Chromosoma       Date:  1976-11-19       Impact factor: 4.316
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