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Literature DB >> 5084792

The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme.

Abstract

Conditions based on previous assays with potassium p-acetylphenyl sulphate have been established for the specific assay of arylsulphatase C in rat tissues. The enzyme has optimum activity with 40mm substrate at pH8.0 in the presence of 0.1m-phosphate buffer. Under these conditions arylsulphatase C can be assayed without interference from the other arylsulphatase enzymes present and is useful as a marker for the endoplasmic reticulum in cell-fractionation studies.

Entities: Chemical Species

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Year: 1972 PMID： 5084792 PMCID： PMC1173768 DOI： 10.1042/bj1280331

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

9 in total

1. Studies on sulphatases. IX. The arylsulphatases of mammalian liver.

Authors: K S DODGSON; B SPENCER; J THOMAS
Journal: Biochem J Date: 1955-01 Impact factor: 3.857

2. Assay of sulfatases.

Authors: K S DODGSON; B SPENCER
Journal: Methods Biochem Anal Date: 1957

3. Studies on sulphatases. 16. A soluble preparation of arysulphatase C of rat-liver microsomes.

Authors: K S DODGSON; F A ROSE; B SPENCER
Journal: Biochem J Date: 1957-06 Impact factor: 3.857

4. The impure nature of nitrocatechol sulphate.

Authors: K S DODGSON; B SPENCER
Journal: Biochim Biophys Acta Date: 1956-07

5. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors: C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal: Biochem J Date: 1955-08 Impact factor: 3.857

6. Studies on sulphatases. 6. The localization of arylsulphatase in the rat-liver cell.

Authors: K S DODGSON; B SPENCER; J THOMAS
Journal: Biochem J Date: 1954-02 Impact factor: 3.857

7. Studies on sulphatases. II. The assay of the arylsulphatase activity of rat tissues.

Authors: K S DODGSON; B SPENCER; J THOMAS
Journal: Biochem J Date: 1953-02 Impact factor: 3.857

8. The sulphatase of ox liver. I. The complex nature of the enzyme.

Authors: A B ROY
Journal: Biochem J Date: 1953-01 Impact factor: 3.857

9. Studies on sulphatases. I. The choice of substrate for the assay of rat-liver arylsulphatase.

Authors: K S DODGSON; B SPENCER
Journal: Biochem J Date: 1953-02 Impact factor: 3.857

9 in total

22 in total

The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme.

1. Studies on sulphatases. IX. The arylsulphatases of mammalian liver.

2. Assay of sulfatases.

3. Studies on sulphatases. 16. A soluble preparation of arysulphatase C of rat-liver microsomes.

4. The impure nature of nitrocatechol sulphate.

5. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

6. Studies on sulphatases. 6. The localization of arylsulphatase in the rat-liver cell.

7. Studies on sulphatases. II. The assay of the arylsulphatase activity of rat tissues.

8. The sulphatase of ox liver. I. The complex nature of the enzyme.

9. Studies on sulphatases. I. The choice of substrate for the assay of rat-liver arylsulphatase.

1. Analysis of iron-containing compounds in different compartments of the rat liver after iron loading.

2. The intracellular distribution of arylsulphatase C and oestrogen sulphatase activities in rat kidney and spleen.

3. Isolation of highly purified rat cerebral lysosomes using percoll gradients with a variety of calcium concentrations.

4. Metabolic fates of diethylstilboestrol sulphates in the rat.

5. There are two distinct arylsulfatase activities in Drosophila.

6. Golgi beta-glucuronidase of androgen-stimulated mouse kidney.

7. Problems associated with the assay of arylsulphatases A and B of rat tissues.

8. Comparative activity of arylsulphatases A and B on two synthetic substrates.

9. Simultaneous azo-coupling method for an estrogen sulfatase in human tissues.

Review 10. Review: the mammalian sulphatases and placental sulphatase deficiency in man.