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Literature DB >> 4723220

Problems associated with the assay of arylsulphatases A and B of rat tissues.

M Worwood, K S Dodgson, G E Hook, F A Rose.

Abstract

A method for the separation and purification of rat liver arylsulphatases A and B by gel filtration on Sephadex G-200 is described. The properties of the A enzyme and its molecular weight are similar to those of the corresponding ox liver enzyme. The B enzymes were found to be dissimilar. The method already developed for the assay of the corresponding enzymes from human tissues was shown to be unsuitable for the assay of the enzymes of rat tissues. A method of assay was developed which permits an approximate determination of the individual rat liver enzymes in a mixture of the two, but precise determination requires prior separation of the enzymes by gel-filtration chromatography.

Entities: Chemical Species

Mesh：

Substances：
Nitrophenols
Sulfatases

Year: 1973 PMID： 4723220 PMCID： PMC1177798 DOI： 10.1042/bj1340183

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

35 in total

7. The effects in the excess administration of prostaglandin E1 on the indicator enzymes of organella membrane in gastric mucosa.

Authors: T Ozeki; K Iwaki; H Masuda; H Ueda
Journal: Br J Exp Pathol Date: 1987-04

Problems associated with the assay of arylsulphatases A and B of rat tissues.

1. EVIDENCE FOR THE GENETIC BLOCK IN METACHROMATIC LEUCODYSTROPHY (ML).

2. The sulphatase of ox liver. 7. The intracellular distribution of sulphatases A and B.

3. Studies on sulphatases. XV. The arylsulphatases of human serum and urine.

4. The impure nature of nitrocatechol sulphate.

5. Mammalian arylsulfatase.

6. The sulphatase of ox liver. II. The purification and properties of sulphatase A.

7. Studies on sulphatases. II. The assay of the arylsulphatase activity of rat tissues.

8. Improvements in the method for the electron microscopic localization of arylsulphatase activity.

9. The cytochemical demonstration of lysosomal aryl sulfatase activity by light and electron microscopy.

10. Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates.

1. Purification and properties of arylsulphatase B of human liver.

2. Arylsulphatase in growing bones of rat.

3. Autophagy-related changes of arylsulphatases A and B in rat liver lysosomes.

4. Relative distribution of arylsulphatases A and B in rat liver parenchymal and other cells.

5. The slow kinetic transients of arylsulphatase A.

6. The effects of vitamin E on the indicator enzymes of organella membranes in the injured liver.

7. The effects in the excess administration of prostaglandin E1 on the indicator enzymes of organella membrane in gastric mucosa.

8. Purification and properties of arylsulphatase A from rabbit testis.

9. Resistance of the insulin crystal to lysosomal proteases: implications for pancreatic B-cell crinophagy.

10. Isolation and characterization of the pig endometrial arylsulphatase A.