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Literature DB >> 4979328

"Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent.

Abstract

The transcription process can in theory be used for genetic mapping on the "intraoperonal" level. We have shown that such an approach, utilizing actinomycin D to create blocks to the travel of the transcription enzyme, is indeed feasible, and the effects of actinomycin D on the synthesis of general pulse-labeled RNA are reported herein.

Entities: Chemical

Mesh：

Substances：

Year: 1969 PMID： 4979328 PMCID： PMC223596 DOI： 10.1073/pnas.63.2.532

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

1. THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D ON THE BIOSYNTHESIS OF ENZYMES IN BACILLUS SUBTILIS AND BACILLUS CEREUS.

Authors: M R POLLOCK
Journal: Biochim Biophys Acta Date: 1963-09-17

2. AMINO ACID ADSORPTION AND PROTEIN SYNTHESIS IN Escherichia Coli.

Authors: R J Britten; R B Roberts; E F French
Journal: Proc Natl Acad Sci U S A Date: 1955-11-15 Impact factor: 11.205

3. Studies of the binding of actinomycin and related compounds to DNA.

Authors: W Müller; D M Crothers
Journal: J Mol Biol Date: 1968-07-28 Impact factor: 5.469

4. The origin and direction of replication of the chromosome of Escherichia coli B-r.

Authors: M L Pato; D A Glaser
Journal: Proc Natl Acad Sci U S A Date: 1968-08 Impact factor: 11.205

5. The formation of 5S ribosomal ribonucleic acid in Bacillus subtilis by posttranscriptional modification.

Authors: N B Hecht; M Bleyman; C R Woese
Journal: Proc Natl Acad Sci U S A Date: 1968-04 Impact factor: 11.205

6. Pyrimidine clusters on the transcribing strand of DNA and their possible role in the initiation of RNA synthesis.

Authors: W Szybalski; H Kubinski; P Sheldrick
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

7. Pulse-labelled RNA formed in the presence of actinomycin D.

Authors: K Kadowaki; B Maruo
Journal: Biochim Biophys Acta Date: 1966-06-22

8. Effects of actinomycin D and 5-fluorouracil on the formation of enzymes in Bacillus subtilis.

Authors: K Kadowaki; J Hosoda; B Maruo
Journal: Biochim Biophys Acta Date: 1965-06-08

9. Relative transcription activity of different segments of the genome throughout the cell division cycle of Escherichia coli. The mapping of ribosomal and transfer RNA and the determination of the direction of replication.

Authors: R G Cutler; J E Evans
Journal: J Mol Biol Date: 1967-05-28 Impact factor: 5.469

7. Study on the reinitiation of transcription in 37 RC cells after actinomycin D inhibition. Spectrum of major RNA species resynthesized after maximal suppression by the drug.

Authors: A Benedetto; C Delfini; G Carloni; W Djaczenko
Journal: J Cell Biol Date: 1975-12 Impact factor: 10.539

7 in total

"Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent.

1. THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D ON THE BIOSYNTHESIS OF ENZYMES IN BACILLUS SUBTILIS AND BACILLUS CEREUS.

2. AMINO ACID ADSORPTION AND PROTEIN SYNTHESIS IN Escherichia Coli.

3. Studies of the binding of actinomycin and related compounds to DNA.

4. The origin and direction of replication of the chromosome of Escherichia coli B-r.

5. The formation of 5S ribosomal ribonucleic acid in Bacillus subtilis by posttranscriptional modification.

6. Pyrimidine clusters on the transcribing strand of DNA and their possible role in the initiation of RNA synthesis.

7. Pulse-labelled RNA formed in the presence of actinomycin D.

8. Effects of actinomycin D and 5-fluorouracil on the formation of enzymes in Bacillus subtilis.

9. Relative transcription activity of different segments of the genome throughout the cell division cycle of Escherichia coli. The mapping of ribosomal and transfer RNA and the determination of the direction of replication.

10. Electrophoretic separation of viral nucleic acids on polyacrylamide gels.

1. Failure of actinomycin D to inhibit the induction of SV 40 viral antigen in BSC 1 transformed cells.

2. Lymphocyte stimulation: a rapid multiparameter analysis.

3. Arrangement and regulation of the nitrogen fixation genes in Klebsiella pneumoniae studied by depression kinetics.

Review 4. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

5. Photosynthetic recovery in the resurrection plant Selaginella lepidophylla after wetting.

6. Formation of all stable RNA species in Escherichia coli by posttranscriptional modification.

7. Study on the reinitiation of transcription in 37 RC cells after actinomycin D inhibition. Spectrum of major RNA species resynthesized after maximal suppression by the drug.