Literature DB >> 822422

Lymphocyte stimulation: a rapid multiparameter analysis.

Z Darzynkiewicz, F Traganos, T Sharpless, M R Melamed.   

Abstract

Several parameters of stimulation of individual lymphocytes are measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.

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Year:  1976        PMID： 822422      PMCID： PMC430788          DOI： 10.1073/pnas.73.8.2881

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

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Authors:  L S LERMAN
Journal:  Proc Natl Acad Sci U S A       Date:  1963-01-15       Impact factor: 11.205

2.  Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.

Authors:  P C NOWELL
Journal:  Cancer Res       Date:  1960-05       Impact factor: 12.701

3.  AGGREGATION OF DYES BOUND TO POLYANIONS.

Authors:  D F Bradley; M K Wolf
Journal:  Proc Natl Acad Sci U S A       Date:  1959-07       Impact factor: 11.205

4.  Suppression of RNA synthesis in lymphocytes by inhibitors of proteolytic enzymes.

Authors:  Z Darzynkiewicz; B G Arnason
Journal:  Exp Cell Res       Date:  1974-03-30       Impact factor: 3.905

5.  Discussion paper: studies on the differentiation within the immune system.

Authors:  J S Haskill
Journal:  Ann N Y Acad Sci       Date:  1973-05-31       Impact factor: 5.691

6.  Microfluorometric characterization of intracellular nucleic acids and nucleoproteins by acridine orange.

Authors:  R Rigler
Journal:  Acta Physiol Scand Suppl       Date:  1966

7.  Effects of inhibition of ribosomal RNA synthesis on the stimulation of lymphocytes by phytohaemagglutinin.

Authors:  J E Kay; B G Leventhal; H L Cooper
Journal:  Exp Cell Res       Date:  1969-01       Impact factor: 3.905

8.  Selective stimulation of IgM synthesis in mouse B lymphocytes by pokeweed mitogen.

Authors:  R M Parkhouse; G Janossy; M F Greaves
Journal:  Nat New Biol       Date:  1972-01-05

Review 9.  Automated cytology.

Authors:  M R Melamed; L A Kamentsky
Journal:  Int Rev Exp Pathol       Date:  1975

10.  Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry.

Authors:  Z Darzynkiewicz; F Traganos; T Sharpless; M R Melamed
Journal:  Exp Cell Res       Date:  1975-10-01       Impact factor: 3.905
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  55 in total

1.  Suppression of C3H/HeJ cell activation by lipopolysaccharide endotoxin.

Authors:  B M Sultzer; J R Bandekar; R Castagna; K Abu-Lawi; M Sadeghian; A J Norin
Journal:  Infect Immun       Date:  1992-09       Impact factor: 3.441

2.  Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes.

Authors:  T Tanaka; M Kajstura; H D Halicka; F Traganos; Z Darzynkiewicz
Journal:  Cell Prolif       Date:  2007-02       Impact factor: 6.831

3.  Activation and proliferation signals in mouse B cells. VI. Anti-Ig antibodies induce dose-dependent cell cycle progression in B cells.

Authors:  G G Klaus; C M Hawrylowicz; C J Carter
Journal:  Immunology       Date:  1985-07       Impact factor: 7.397

4.  RNA recovery efficiency for cultured cells and organ-derived cell suspensions.

Authors:  E S Berleth; B J Dolnick; M J Ehrke; E Mihich
Journal:  Nucleic Acids Res       Date:  1994-05-25       Impact factor: 16.971

5.  B cell hyperactivity in autoimmune continuous B cell lines.

Authors:  M Aldo-Benson; M S Brooks; L Scheiderer-Pratt
Journal:  Immunol Res       Date:  1989       Impact factor: 2.829

6.  Cell cycle regulation by growth factors and nutrients in normal and transformed cells.

Authors:  D Paul; K D Brown; H T Rupniak; H J Ristow
Journal:  In Vitro       Date:  1978-01

7.  Wheat germ agglutinin-positive cells in a stem cell-enriched fraction of mouse bone marrow have potent natural suppressor activity.

Authors:  K Sugiura; M Inaba; H Ogata; R Yasumizu; K Inaba; R A Good; S Ikehara
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

8.  Expression of the p12 subunit of human DNA polymerase δ (Pol δ), CDK inhibitor p21(WAF1), Cdt1, cyclin A, PCNA and Ki-67 in relation to DNA replication in individual cells.

Authors:  Hong Zhao; Sufang Zhang; Dazhong Xu; Marietta Ywt Lee; Zhongtao Zhang; Ernest Yc Lee; Zbigniew Darzynkiewicz
Journal:  Cell Cycle       Date:  2014       Impact factor: 4.534

9.  Cell cycle arrest during measles virus infection: a G0-like block leads to suppression of retinoblastoma protein expression.

Authors:  D Naniche; S I Reed; M B Oldstone
Journal:  J Virol       Date:  1999-03       Impact factor: 5.103

10.  Effect of swainsonine on interleukin-2 alpha chain receptor expression and proliferation of human lymphocytes.

Authors:  A Myc; P DeAngelis; P Lassota; M R Melamed; Z Darzynkiewicz
Journal:  Clin Exp Immunol       Date:  1991-06       Impact factor: 4.330
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