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Literature DB >> 4975741

Preparation of cell walls and protoplasm of Neisseria with the Ribi cell fractionator.

J E Martin, W L Peacock, G Reising, D S Kellogg, E Ribi, J D Thayer.

Abstract

The varied pressures required for disruption of Neisseria gonorrhoeae and other species of Neisseria when the Sorvall-Ribi refrigerated cell fractionator is used in the preparation of cell walls and cellular protoplasm are reported. Optimal disruption pressure for the gonococcus was considerably less than that required for other members of the genus Neisseria. Pressures varied from 8,000 psi for N. gonorrhoeae F62, colony type 4, to 22,000 psi for the nonpathogenic Neisseria-N. sicca, N. flava, and N. catarrhalis. Representative electron photomicrographs are shown.

Entities: Disease Species

Mesh：

Year: 1969 PMID： 4975741 PMCID： PMC249806 DOI： 10.1128/jb.97.3.1009-1011.1969

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

8 in total

1. ENZYMES RELEASED FROM ESCHERICHIA COLI WITH THE AID OF A SERVALL CELL FRACTIONATOR.

Authors: J A DUERRE; E RIBI
Journal: Appl Microbiol Date: 1963-11

2. Reiter protein complement fixation test for syphilis.

Authors: G R CANNEFAX; W GARSON
Journal: Public Health Rep Date: 1957-04 Impact factor: 2.792

3. The Killing of Bacterial Spores in Fluids by Agitation with Small Inert Particles.

Authors: H R Curran; F R Evans
Journal: J Bacteriol Date: 1942-02 Impact factor: 3.490

4. Active Enzyme Preparations from Bacteria.

Authors: G Kalnitsky; M F Utter; C H Werkman
Journal: J Bacteriol Date: 1945-06 Impact factor: 3.490

5. Ultrasonic Disintegration as Method of Extracting Bacterial Enzymes.

Authors: P K Stumpf; D E Green; F W Smith
Journal: J Bacteriol Date: 1946-04 Impact factor: 3.490

6. Colloidal dispersion of chloroplast material.

Authors: H W MILNER; N S LAWRENCE; C S FRENCH
Journal: Science Date: 1950-06-09 Impact factor: 47.728

7. NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD.

Authors: L A WHITE; D S KELLOGG
Journal: Appl Microbiol Date: 1965-03

8. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.

Authors: D S KELLOGG; W L PEACOCK; W E DEACON; L BROWN; D I PIRKLE
Journal: J Bacteriol Date: 1963-06 Impact factor: 3.490

8 in total

9 in total

9. Antigens of Neisseria gonorrhoeae: characterization by gel filtration, complement fixation, and agar-gel diffusion of antigens of gonococcal protoplasm.

Authors: D G Danielsson; J D Schmale; W L Peacock; J D Thayer
Journal: J Bacteriol Date: 1969-03 Impact factor: 3.490

9 in total

Preparation of cell walls and protoplasm of Neisseria with the Ribi cell fractionator.

1. ENZYMES RELEASED FROM ESCHERICHIA COLI WITH THE AID OF A SERVALL CELL FRACTIONATOR.

2. Reiter protein complement fixation test for syphilis.

3. The Killing of Bacterial Spores in Fluids by Agitation with Small Inert Particles.

4. Active Enzyme Preparations from Bacteria.

5. Ultrasonic Disintegration as Method of Extracting Bacterial Enzymes.

6. Colloidal dispersion of chloroplast material.

7. NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD.

8. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.

1. Particulate Fractions of Neisseria gonorrhoeae.

2. Chemical characterization of organisms isolated from leprosy patients.

3. An automated complement fixation procedure for detecting antibody to N. gonorrhoeae.

4. Human antibody response to lipopolysaccharides from Neisseria gonorrhoeae.

5. Reactivity of two selected antigens of Neisseria gonorrhoeae.

6. Isolation of an antigen of Neisseria gonorrhoeae involved in the human immune response to gonococcal infection.

7. Lymphocyte transformation in repeated gonococcal urethritis.

8. Nature and Heterogeneity of the Antigens of Neisseria gonorrhoeae Involved in the Serum Bactericidal Reaction.

9. Antigens of Neisseria gonorrhoeae: characterization by gel filtration, complement fixation, and agar-gel diffusion of antigens of gonococcal protoplasm.