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Literature DB >> 4971885

Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin.

W H Coleman, J Kaur, M E Iwert, G J Kasai, W Burrows.

Abstract

Details for the preparation and partial purification of culture supernatant fluids of Vibrio cholerae (V. comma) 569B which retain rabbit ileal loop fluid-accumulating activity are presented. These preparations were fractionated on Sephadex G-200 and on diethylaminoethyl-Sephadex. On the latter, two fractions were obtained by elution with a linear sodium chloride gradient. The fraction designated "fraction I" retains the toxic activity as demonstrated in the rabbit ileal loop model. Chemical and immunological properties of this active fraction are described.

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Year: 1968 PMID： 4971885 PMCID： PMC252427 DOI： 10.1128/jb.96.4.1137-1143.1968

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

1. Enterotoxicity of bacteria-free culture-filtrate of Vibrio cholerae.

Authors: S N DE
Journal: Nature Date: 1959-05-30 Impact factor: 49.962

2. Role of cholera a toxin in experimental cholera.

Authors: N K DUTTA; M V PANSE; D R KULKARNI
Journal: J Bacteriol Date: 1959-10 Impact factor: 3.490

3. [A micro-method of immuno-electrophoresis].

Authors: J J SCHEIDEGGER
Journal: Int Arch Allergy Appl Immunol Date: 1955

4. Diffusion-in-gel methods for immunological analysis. II.

Authors: O OUCHTERLONY
Journal: Prog Allergy Date: 1962

5. Increased serum glutamic-oxalacetic transaminase and lactic dehydrogenase levels produced by cholera infection and cell-free toxin in the rabbit.

Authors: M E Iwert; G J Leitch; W Burrows
Journal: J Infect Dis Date: 1967-06 Impact factor: 5.226

Review 6. The pathophysiology of cholera.

Authors: T R Hendrix
Journal: Bull N Y Acad Med Date: 1971-10

Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin.

1. Enterotoxicity of bacteria-free culture-filtrate of Vibrio cholerae.

2. Role of cholera a toxin in experimental cholera.

3. [A micro-method of immuno-electrophoresis].

4. Diffusion-in-gel methods for immunological analysis. II.

5. Increased serum glutamic-oxalacetic transaminase and lactic dehydrogenase levels produced by cholera infection and cell-free toxin in the rabbit.

6. Fatty acid composition of bacterial membrane and wall lipids.

7. The titration of cholera toxin and antitoxin in the rabbit ileal loop.

8. Cholera infection and toxin in the rabbit ileal loop.

9. Site and characteristics of electrolyte loss and effect of intraluminal glucose in experimental canine cholera.

10. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

1. Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin.

2. Immunochemical Studies of Two Cholera Toxin-Containing Standard Culture Filtrate Preparations of Vibrio cholerae.

3. Biochemistry of Vibrio cholerae virulence: purification of cholera enterotoxin by preparative disc electrophoresis.

4. Simple method for purifying choleragenoid, the natural toxoid of Vibrio cholerae.

Review 5. Vibrio cholerae enterotoxin and its mode of action.

Review 6. The pathophysiology of cholera.

7. Immunity to cholera: relation of fraction II of type 2 cholera toxin to vibriocidal antibody.

8. Titration of antibody to soluble antigens of the cholera vibrios by passive haemagglutination.

9. Reaction of Vibrio cholerae and choleragenic toxin in ileal loop of laboratory animals.

10. Factors influencing in vitro skin permeability factor production by Vibrio cholerae.