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Literature DB >> 16557774

Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin.

Abstract

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Although not completely free from traces of vibriocidal antibody-stimulating activity, the resultant purified toxic immunogen appeared homogeneous as judged by immunoelectrophoresis, immunodiffusion, and disc electrophoresis. Advantages and disadvantages of this purification method are discussed in comparison with those of previously published techniques.

Entities: Chemical Disease

Year: 1970 PMID： 16557774 PMCID： PMC415940 DOI： 10.1128/iai.1.6.546-554.1970

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

7 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. Factors influencing in vitro skin permeability factor production by Vibrio cholerae.

Authors: S H Richardson
Journal: J Bacteriol Date: 1969-10 Impact factor: 3.490

3. Isolation of cholera toxins by dextran sulfate precipitation.

Authors: S H Richardson; D J Evans
Journal: J Bacteriol Date: 1968-10 Impact factor: 3.490

4. In vitro production of choleragen and vascular permeability factor by Vibrio cholerae.

Authors: D J Evans; S H Richardson
Journal: J Bacteriol Date: 1968-07 Impact factor: 3.490

5. A permeability factor (toxin) found in cholera stools and culture filtrates and its neutralization by convalescent cholera sera.

Authors: J P Craig
Journal: Nature Date: 1965-08-07 Impact factor: 49.962

6. Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin.

Authors: W H Coleman; J Kaur; M E Iwert; G J Kasai; W Burrows
Journal: J Bacteriol Date: 1968-10 Impact factor: 3.490

7. Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid.

Authors: R A Finkelstein; J J LoSpalluto
Journal: J Exp Med Date: 1969-07-01 Impact factor: 14.307

7 in total

8 in total

1. Biochemistry of Vibrio cholerae virulence: purification of cholera enterotoxin by preparative disc electrophoresis.

Authors: A C Lewis; S H Richardson; B Sheridan
Journal: Appl Environ Microbiol Date: 1976-08 Impact factor: 4.792

Review 2. Vibrio cholerae enterotoxin and its mode of action.

Authors: N F Pierce; W B Greenough; C C Carpenter
Journal: Bacteriol Rev Date: 1971-03

3. Experimental studies on cholera immunization. II. Evidence for protective antitoxic immunity mediated by serum antibodies as well as local antibodies.

Authors: J Holmgren; A Andersson; G Wallerstrom; O Ouchterlony
Journal: Infect Immun Date: 1972-05 Impact factor: 3.441

4. Biochemistry of Vibrio cholerae virulence. 3. Nutritional requirements for toxin production and the effects of pH on toxin elaboration in chemically defined media.

Authors: L T Callahan; S H Richardson
Journal: Infect Immun Date: 1973-04 Impact factor: 3.441

8. Biochemistry of vibrio cholerae virulence. II. Skin permeability factor-cholera enterotoxin production in a chemically defined medium.

Authors: L T Callahan; R C Ryder; S H Richardson
Journal: Infect Immun Date: 1971-11 Impact factor: 3.441