Literature DB >> 4947395

Ribonucleic acid-deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide.

S Gillespie, D Gillespie.   

Abstract

Hybridization in 6xSSC (SSC, 0.15m-sodium chloride-0.015m-sodium citrate) at 66 degrees C was compared with hybridization in formamide-6xSSC (1:1, v/v) at 35 degrees C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridization reaction were explored and it was concluded that the formamide system is generally superior.

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Year:  1971        PMID： 4947395      PMCID： PMC1178082          DOI： 10.1042/bj1250481

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  6 in total

1.  Determination of the amount of DNA on nitrocellulose mebrane filters.

Authors:  W H. Meijs; R A. Schilperoort
Journal:  FEBS Lett       Date:  1971-01-12       Impact factor: 4.124

2.  A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate.

Authors:  W C Summers
Journal:  Anal Biochem       Date:  1970-02       Impact factor: 3.365

3.  Metabolic events occurring during recovery from prolonged glucose starvation in Escherichia coli.

Authors:  A Jacobson; D Gillespie
Journal:  J Bacteriol       Date:  1968-03       Impact factor: 3.490

4.  A method for the hybridization of nucleic acid molecules at low temperature.

Authors:  J Bonner; G Kung; I Bekhor
Journal:  Biochemistry       Date:  1967-12       Impact factor: 3.162

5.  A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.

Authors:  D Gillespie; S Spiegelman
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

6.  Nucleic acid reassociation in formamide.

Authors:  B L McConaughy; C D Laird; B J McCarthy
Journal:  Biochemistry       Date:  1969-08       Impact factor: 3.162
  6 in total
  28 in total

1.  Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells.

Authors:  J B Clements; R L Sinsheimer
Journal:  J Virol       Date:  1975-01       Impact factor: 5.103

2.  Analysis of the Symbiotic Performance of Bradyrhizobium japonicum USDA 110 and Its Derivative I-110 and Discovery of a New Mannitol-Utilizing, Nitrogen-Fixing USDA 110 Derivative.

Authors:  J N Mathis; D W Israel; W M Barbour; B D Jarvis; G H Elkan
Journal:  Appl Environ Microbiol       Date:  1986-07       Impact factor: 4.792

3.  Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium.

Authors:  H Huber; K O Stetter
Journal:  Appl Environ Microbiol       Date:  1990-02       Impact factor: 4.792

4.  Detection of messenger RNA from the isoleucine--valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: involvement of transfer RNA in transcriptional repression.

Authors:  G Childs; F Sonnenberg; M Freundlich
Journal:  Mol Gen Genet       Date:  1977-03-07

5.  Chloramphenicol-induced changes in the synthesis of ribosomal, transfer, and messenger ribonucleic acids in Escherichia coli B/r.

Authors:  V Shen; H Bremer
Journal:  J Bacteriol       Date:  1977-06       Impact factor: 3.490

6.  Loss of rRNA genes during a phenotypic, masculinizing mutation in Allomyces arbuscla.

Authors:  M Ojha; G Turian
Journal:  Mol Gen Genet       Date:  1978-10-30

7.  Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs.

Authors:  L M Hsu; H J Klee; J Zagorski; M J Fournier
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

8.  Stimulation of RNA synthesis by two protein factors in extracts of Escherichia coli.

Authors:  Y Murooka; R A Lazzarini
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

9.  Intracellular forms of Adenovirus DNA. II. Isolation in dye-buoyant density gradients of a DNA-RNA complex from KB cells infected with Adenovirus type 2.

Authors:  W Doerfler; U Lundholm; U Rensing; L Philipson
Journal:  J Virol       Date:  1973-10       Impact factor: 5.103

10.  Systematics of RNA tumor viruses and virus-like particles of human origin.

Authors:  N R Miller; W C Saxinger; M S Reitz; R E Gallagher; A M Wu; R C Gallo; D Gillespie
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205
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