Literature DB >> 4943786

Purification of the araC protein.

G Wilcox, K J Clemetson, D V Santi, E Englesberg.   

Abstract

The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.

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Year:  1971        PMID： 4943786      PMCID： PMC389371          DOI： 10.1073/pnas.68.9.2145

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI.

Authors:  C WILLSON; D PERRIN; M COHN; F JACOB; J MONOD
Journal:  J Mol Biol       Date:  1964-04       Impact factor: 5.469

2.  Mechanism of activation of catabolite-sensitive genes: a positive control system.

Authors:  G Zubay; D Schwartz; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1970-05       Impact factor: 11.205

3.  Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r.

Authors:  J Irr; E Englesberg
Journal:  Genetics       Date:  1970-05       Impact factor: 4.562

4.  Lac repressor-operator interaction. I. Equilibrium studies.

Authors:  A D Riggs; H Suzuki; S Bourgeois
Journal:  J Mol Biol       Date:  1970-02-28       Impact factor: 5.469

5.  Further evidence for positive control of the L-arabinose system by gene araC.

Authors:  D E Sheppard; E Englesberg
Journal:  J Mol Biol       Date:  1967-05-14       Impact factor: 5.469

6.  The purification of beta-galactosidase from Escherichia coli by affinity chromatography.

Authors:  E Steers; P Cuatrecasas; H B Pollard
Journal:  J Biol Chem       Date:  1971-01-10       Impact factor: 5.157

7.  L-arabinose binding protein from Escherichia coli B-r.

Authors:  R W Hogg; E Englesberg
Journal:  J Bacteriol       Date:  1969-10       Impact factor: 3.490

8.  D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC.

Authors:  S Beverin; D E Sheppard; S S Park
Journal:  J Bacteriol       Date:  1971-07       Impact factor: 3.490

9.  Positive control of enzyme synthesis by gene C in the L-arabinose system.

Authors:  E Englesberg; J Irr; J Power; N Lee
Journal:  J Bacteriol       Date:  1965-10       Impact factor: 3.490

10.  Hyperinducibility as a result of mutation in structural genes and self-catabolite repression in the ara operon.

Authors:  L Katz; E Englesberg
Journal:  J Bacteriol       Date:  1971-07       Impact factor: 3.490
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  9 in total

1.  Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

Authors:  L G Cass; G Wilcox
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

2.  Design of molecular control mechanisms and the demand for gene expression.

Authors:  M A Savageau
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

3.  Genetic regulatory mechanisms and the ecological niche of Escherichia coli.

Authors:  M A Savageau
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

4.  The araC regulatory gene mRNA contains a leader sequence.

Authors:  L G Cass; A H Horwitz; C G Miyada; L Greenfield; G Wilcox
Journal:  Mol Gen Genet       Date:  1980

Review 5.  Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

Authors:  N R Pace
Journal:  Bacteriol Rev       Date:  1973-12

6.  Transcriptional control in the L-arabinose operon of Escherichia coli B-r.

Authors:  P P Cleary; E Englesberg
Journal:  J Bacteriol       Date:  1974-04       Impact factor: 3.490

7.  lac repressor: 3-fluorotyrosine substitution for nuclear magnetic resonance studies.

Authors:  P Lu; M Jarema; K Mosser; W E Daniel
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

8.  Characterization of strong polar mutations in a region immediately adjacent to the L-arabinose operator in Escherichia coli B-r.

Authors:  M Eleuterio; B Griffin; D E Sheppard
Journal:  J Bacteriol       Date:  1972-08       Impact factor: 3.490

9.  The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r.

Authors:  R Bass; L Heffernan; K Sweadner; E Englesberg
Journal:  Arch Microbiol       Date:  1976-10-11       Impact factor: 2.552
  9 in total

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