Literature DB >> 49343

Purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization.

W D Kuhlmann.   

Abstract

Mouse alpha1-fetoprotein (AFP) was isolated from amniotic fluid by immunoadsorbent columns and preparative electrophoresis. Specific antibodies were isolated from monospecific hyperimmunsera by use of immunoadsorbents, and subsequently coupled with horseradish peroxidase. At the light microscopical level, purified antibody-peroxidase conjugates were used for the cellular localization of AFP in fetal liver by direct and indirect staining methods. Fixatives containing ethanol or aldehydes were tried for antigen staining. Prior to immunocytological reactions, endogenous peroxidases were inhibited by hydrogen peroxide.

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Year:  1975        PMID： 49343     DOI： 10.1007/bf00494077

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  35 in total

1.  Purification and chemical characterization of alpha-fetoprotein from rat and mouse.

Authors:  H Watabe
Journal:  Int J Cancer       Date:  1974-03-15       Impact factor: 7.396

2.  A comparative study for ultrastructural localization of intracellular immunoglobulins using peroxidase conjugates.

Authors:  W D Kuhlmann; S Avrameas; T Ternynck
Journal:  J Immunol Methods       Date:  1974-05       Impact factor: 2.303

3.  Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.

Authors:  J G Streefkerk
Journal:  J Histochem Cytochem       Date:  1972-10       Impact factor: 2.479

4.  [Alpha 1 -foetoprotein. Isolation and crystallization from human plasma].

Authors:  F G Lehmann; D Lehmann; G A Martini
Journal:  Clin Chim Acta       Date:  1971-06       Impact factor: 3.786

Review 5.  Immunoenzyme techniques: enzymes as markers for the localization of antigens and antibodies.

Authors:  S Avrameas
Journal:  Int Rev Cytol       Date:  1970

6.  [The appearance of embryonic serum alpha-globulin in adult mice following carbon tetrachloride poisoning].

Authors:  R D Bakirov
Journal:  Biull Eksp Biol Med       Date:  1968-02

7.  [Transitory cell antigens of rat liver. I. The secretion and synthesis of fetospecific serum proteins during hepatic development and regeneration].

Authors:  B de Néchaud; J Uriel
Journal:  Int J Cancer       Date:  1971-07-15       Impact factor: 7.396

8.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

9.  Effect of formaldehyde, glutaraldehyde and sucrose on the tissue antigenicity.

Authors:  J S Deng; E H Beutner
Journal:  Int Arch Allergy Appl Immunol       Date:  1974

10.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479
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  16 in total

1.  Immunohistochemical studies on human gastric mucosa. Procedures for routine demonstration of gastric proteins by immunoenzyme techniques.

Authors:  K Wurster; W D Kuhlmann; W Rapp
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1978-06-15

2.  Glucose oxidase as label in histological immunoassays with enzyme-amplification in a two-step technique: coimmobilized horseradish peroxidase as secondary system enzyme for chromogen oxidation.

Authors:  W D Kuhlmann; P Peschke
Journal:  Histochemistry       Date:  1986

3.  Comparative study of procedures for histological detection of lectin binding by use of Griffonia simplicifolia agglutinin I and gastrointestinal mucosa of the rat.

Authors:  W D Kuhlmann; P Peschke
Journal:  Histochemistry       Date:  1984

4.  Histological detection of lectin binding sites in human gastrointestinal mucosa.

Authors:  P Peschke; W D Kuhlmann; K Wurster
Journal:  Experientia       Date:  1983-03-15

5.  Immunocytochemical localization of progesterone-binding protein (PBP) in guinea-pig placental tissue.

Authors:  M Perrot-Applanat; J F David-Ferreira
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

6.  Immunohistochemical localization of alpha 1-antitrypsin in normal mouse liver and pancreas.

Authors:  J Gauldie; L Lamontagne; P Horsewood; E Jenkins
Journal:  Am J Pathol       Date:  1980-12       Impact factor: 4.307

7.  Microarray analysis reveals altered circulating microRNA expression in mice infected with Coxsackievirus B3.

Authors:  Chaoyu Sun; Lei Tong; Wenran Zhao; Yan Wang; Yuan Meng; Lexun Lin; Bingchen Liu; Yujia Zhai; Zhaohua Zhong; Xueqi Li
Journal:  Exp Ther Med       Date:  2016-08-22       Impact factor: 2.447

8.  Diaminobenzidine cytochemistry in cryo-ultramicrotomy for the detection of peroxidases.

Authors:  W D Kuhlmann; A Viron
Journal:  Histochemistry       Date:  1977-12-28

9.  Correlation of histology and alpha 1-fetoprotein resurgence in rat liver regeneration after experimental injury by galactosamine.

Authors:  W D Kuhlmann; K Wurster
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1980

10.  Cellular localization of lectin-affinity in tissue sections of normal human duodenum.

Authors:  K Wurster; P Peschke; W D Kuhlmann
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1983
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