Literature DB >> 340431

Diaminobenzidine cytochemistry in cryo-ultramicrotomy for the detection of peroxidases.

W D Kuhlmann, A Viron.   

Abstract

Rat erythrocytes and lymphoid cells were fixed by glutaraldehyde and encapsulated in bovine serum albumin plus rabbit IgG globulins for cryo-ultramicrotomy. A technical procedure is described by which endogenous peroxidases of erythrocytes in ultrathin frozen sections were detected by hydrogen peroxide and diaminobenzidine as hydrogen donor. Modifications of this classical cytochemical procedure proved also useful in cryoultramicrotomy for immunoperoxidase labeling of antigenic determinants in rabbit IgG globulins which have been crosslinked within the supporting matrix of cells.

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Year:  1977        PMID： 340431     DOI： 10.1007/bf00508275

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  11 in total

1.  A comparative study for ultrastructural localization of intracellular immunoglobulins using peroxidase conjugates.

Authors:  W D Kuhlmann; S Avrameas; T Ternynck
Journal:  J Immunol Methods       Date:  1974-05       Impact factor: 2.303

2.  Ultracryotomy: a routine procedure.

Authors:  J R Iglesias; R Bernier; R Simard
Journal:  J Ultrastruct Res       Date:  1971-07

3.  A comparative study of the techniques for ultrastructural localization of antienzyme antibodies.

Authors:  W D Kuhlmann; H R Miller
Journal:  J Ultrastruct Res       Date:  1971-05

4.  Cross-linked albumin as supporting matrix in ultrathin cryo microtomy.

Authors:  W D Kuhlmann; A Viron
Journal:  J Ultrastruct Res       Date:  1972-11

5.  Ultrastructural immunoperoxidase cytochemistry.

Authors:  W D Kuhlmann
Journal:  Prog Histochem Cytochem       Date:  1977

6.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

7.  Purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization.

Authors:  W D Kuhlmann
Journal:  Histochemistry       Date:  1975-07-30

8.  Improved procedures for immunoferritin labeling of ultrathin frozen sections.

Authors:  K T Tokuyasu; S J Singer
Journal:  J Cell Biol       Date:  1976-12       Impact factor: 10.539

9.  Improved techniques for the preparation of ultrathin frozen sections.

Authors:  W Bernhard; A Viron
Journal:  J Cell Biol       Date:  1971-06       Impact factor: 10.539

10.  Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.

Authors:  D D SABATINI; K BENSCH; R J BARRNETT
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539
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  2 in total

1.  Advances in ultrastructural postembedment localization of antigens in Epon sections with peroxidase labelled antibodies.

Authors:  W D Kuhlmann; P Peschke
Journal:  Histochemistry       Date:  1982

2.  Conditional ablation of macrophages disrupts ovarian vasculature.

Authors:  Emily C Turner; Jeremy Hughes; Helen Wilson; Michael Clay; Katie J Mylonas; Tiina Kipari; W Colin Duncan; Hamish M Fraser
Journal:  Reproduction       Date:  2011-03-10       Impact factor: 3.906
  2 in total

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