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Literature DB >> 4933471

Studies of membrane formation in Tetrahymena pyriformis. II. Isolation and lipid analysis of cell fractions.

Abstract

A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.

Entities: Chemical Species

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Year: 1971 PMID： 4933471 PMCID： PMC2108490 DOI： 10.1083/jcb.49.3.712

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

21 in total

1. Phosphorus assay in column chromatography.

Authors: G R BARTLETT
Journal: J Biol Chem Date: 1959-03 Impact factor: 5.157

2. Respiratory enzyme studies in Tetrahymena pyriformis. 4. Stabilization of electron-transport components.

Authors: H J EICHEL
Journal: Biochem J Date: 1959-01 Impact factor: 3.857

3. A simple method for the isolation and purification of total lipides from animal tissues.

Authors: J FOLCH; M LEES; G H SLOANE STANLEY
Journal: J Biol Chem Date: 1957-05 Impact factor: 5.157

4. Studies of membrane formation in Tetrahymena pyriformis. I. Rates of phospholipid biosynthesis.

Authors: G A Thompson
Journal: Biochemistry Date: 1967-07 Impact factor: 3.162

5. The chromatographic separation of phosphonolipids from their phospholipid analogs.

Authors: V M Kapoulas
Journal: Biochim Biophys Acta Date: 1969-03-04

6. Ribonucleic acid synthesis in isolated mitochondria from Tetrahymena.

Authors: Y Suyama; J Eyer
Journal: J Biol Chem Date: 1968-01-25 Impact factor: 5.157

7. The properties of an enzyme system degrading endogenous phospholipids of Tetrahymena pyriformis.

Authors: G A Thompson
Journal: J Protozool Date: 1969-08

8. Fine structure, reconstruction and possible functions of components of the cortex of Tetrahymena pyriformis.

Authors: R D Allen
Journal: J Protozool Date: 1967-11

9. Heterogeneity of rough-surfaced liver microsomal membranes of adult, phenobarbital-treated, and newborn rats.

Authors: G Dallner; A Bergstrand; R Nilsson
Journal: J Cell Biol Date: 1968-08 Impact factor: 10.539

10. Studies of membrane formation in Tetrahymena pyriformis. II. Isolation and lipid analysis of cell fractions.

Authors: Y Nozawa; G A Thompson
Journal: J Cell Biol Date: 1971-06 Impact factor: 10.539

20 in total

1. The steric requirements for sterol inhibition of tetrahymanol biosynthesis.

Authors: R L Conner; J R Landrey
Journal: Lipids Date: 1978-10 Impact factor: 1.880

2. Altered microviscosity of in vivo lipid-manipulated membranes in Tetrahymena pyriformis: a fluorescence study.

Authors: H Shimonaka; H Fukushima; K Kawai; S Nagao; Y Okano; Y Nozawa
Journal: Experientia Date: 1978-05-15

3. Presence of benzodiazepine binding sites (receptors) and amplification thereof by imprinting in Tetrahymena.

Authors: G Csaba; A K Fülöp; A Inczefi-Gonda
Journal: Experientia Date: 1989-01-15

4. An apparent association between glycosylphosphatidylinositol-anchored proteins and a sphingolipid in Tetrahymena mimbres.

Authors: X Zhang; G A Thompson
Journal: Biochem J Date: 1997-04-01 Impact factor: 3.857

10. Modification of microsomal lipid composition and electron transport enzyme activities in isovalerate-supplemented cells of novel Tetrahymena ISO.

Authors: T Watanabe; H Fukushima; N Sasaki; S Umeki; Y Suezawa; Y Nozawa
Journal: Lipids Date: 1983-06 Impact factor: 1.880