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Literature DB >> 4910848

A mutant of Escherichia coli with temperature-sensitive streptomycin protein.

Abstract

The temperature-sensitive mutation in our Escherichia coli strain C1714 appears to be in a ribosomal protein. The mutation maps at the streptomycin locus. Protein synthesis in intact cells stops immediately when the temperature is shifted from 30 to 42 degrees C. Analysis of polyribosome distributions after shift-up suggests that initiation of protein synthesis is defective at the high temperature. In vitro protein synthesis in extracts from C1714 is temperature sensitive when directed by natural mRNA, but not when directed by poly U.

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Year: 1970 PMID： 4910848 PMCID： PMC282941 DOI： 10.1073/pnas.65.3.544

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

12 in total

8. Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis.

Authors: L Luzzatto; D Apirion; D Schlessinger
Journal: Proc Natl Acad Sci U S A Date: 1968-07 Impact factor: 11.205

10. Restoration by ribosomal protein S1 of the defective translation in a temperature-sensitive mutant of Escherichia coli K-12: characterization and genetic studies.

Authors: H Ohsawa; B Maruo
Journal: J Bacteriol Date: 1976-09 Impact factor: 3.490

A mutant of Escherichia coli with temperature-sensitive streptomycin protein.

1. STUDIES ON THE RIBOSOMES OF STREPTOMYCIN-SENSITIVE AND RESISTANT STRAINS OF ESCHERICHIA COLI.

2. ON THE COLINEARITY OF GENE STRUCTURE AND PROTEIN STRUCTURE.

3. Ribosomal localization of streptomycin sensitivity.

4. Inhibition of polypeptide synthesis by streptomycin.

5. Transduction of linked genetic characters of the host by bacteriophage P1.

6. In vitro synthesis of T4 proteins.

7. Rapid inhibition of polypeptide chain extension by streptomycin.

8. Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis.

9. Polyribosomes of growing bacteria.

10. Cell-free protein synthesis programmed with R17 RNA: identification of two phage proteins.

1. Synthesis of Specific, Stabilized Messenger RNA When Translocation Is Blocked in ESCHERICHIA COLI.

2. A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis.

3. A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein.

4. Activity of colicin E3-treated ribosomes in initiation and in chain elongation.

5. A new type of suppression of temperature sensitive gene activity in Escherichia coli.

6. Temperature-sensitive relaxed Phenotype in a stringent strain of Escherichia coli.

7. Continuous requirement for host P10 protein during bacteriophage T4 infection.

8. Physiological properties of the cladosarum-like mutant 22B of Aspergillus aureolatus.

9. Increased loss of duplicated genes in streptomycin-resistant (strA) mutants of Escherichia coli k-12.

10. Restoration by ribosomal protein S1 of the defective translation in a temperature-sensitive mutant of Escherichia coli K-12: characterization and genetic studies.