Literature DB >> 340905

A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein.

L A Isaksson, S E Sköld, J Skjöldebrand, R Takata.   

Abstract

A procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli has been developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible lambda prophage and either streptomycin plus ampillicin or ampicillin plus cycloserine. The mutants so obtained are blocked in vivo in the synthesis of RNA or protein or both at restrictive temperature.

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Year:  1977        PMID: 340905     DOI: 10.1007/bf00267177

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  27 in total

1.  Damage by streptomycin to the cell membrane of Escherichia coli.

Authors:  N ANAND; B D DAVIS
Journal:  Nature       Date:  1960-01-02       Impact factor: 49.962

2.  Mutant E. coli strain with temperature sensitive peptidyl-transfer RNA hydrolase.

Authors:  A G Atherly; J R Menninger
Journal:  Nat New Biol       Date:  1972-12-20

3.  Metabolism of guanosine tetraphosphate in Escherichia coli.

Authors:  E Lund; N O Kjeldgaard
Journal:  Eur J Biochem       Date:  1972-07-24

4.  Mutants of Escherichia coli blocked in protein synthesis: mutants with an altered G factor.

Authors:  G P Tocchini-Valentini; L Felicetti; G M Rinaldi
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

5.  Altered S5 and S20 ribosomal proteins in revertants of an alanyl-tRNA synthetase mutant of Escherichia coli.

Authors:  H G Wittmann; G Stöffler
Journal:  Mol Gen Genet       Date:  1974

6.  Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA.

Authors:  G R Björk; L A Isaksson
Journal:  J Mol Biol       Date:  1970-07-14       Impact factor: 5.469

7.  Resistance of Escherichia coli to penicillins. II. An improved mapping of the ampA gene.

Authors:  K G Eriksson-Grennberg
Journal:  Genet Res       Date:  1968-10       Impact factor: 1.588

8.  Studies on the RNA polymerase in Escherichia coli K12 using the mutation affecting its activity.

Authors:  R B Khesin; M F Shemyakin; A M Gorlenko; S Z Mindlin; T S Ilyina
Journal:  J Mol Biol       Date:  1969-06-28       Impact factor: 5.469

9.  A mutant of Escherichia coli with an altered elongation factor Tu.

Authors:  S Pedersen; R M Blumenthal; S Reeh; L B Russell; P Lemaux; R A Laursen; S Nagarkatti; J D Friesen
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

10.  A mutant of Escherichia coli with temperature-sensitive streptomycin protein.

Authors:  S S Kang
Journal:  Proc Natl Acad Sci U S A       Date:  1970-03       Impact factor: 11.205

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  28 in total

1.  sigma(70) is the principal sigma factor responsible for transcription of acs, which encodes acetyl coenzyme A synthetase in Escherichia coli.

Authors:  S Kumari; E J Simel; A J Wolfe
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

2.  Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli.

Authors:  Thomas Kruse; Blagoy Blagoev; Anders Løbner-Olesen; Masaaki Wachi; Kumi Sasaki; Noritaka Iwai; Matthias Mann; Kenn Gerdes
Journal:  Genes Dev       Date:  2006-01-01       Impact factor: 11.361

3.  Regulation of igaA and the Rcs system by the MviA response regulator in Salmonella enterica.

Authors:  Clara B García-Calderón; Josep Casadesús; Francisco Ramos-Morales
Journal:  J Bacteriol       Date:  2009-02-13       Impact factor: 3.490

4.  Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2.

Authors:  G E Christie; S B Cale; L A Isaksson; D J Jin; M Xu; B Sauer; R Calendar
Journal:  J Bacteriol       Date:  1996-12       Impact factor: 3.490

5.  DsbA and MgrB regulate steA expression through the two-component system PhoQ/PhoP in Salmonella enterica.

Authors:  Elena Cardenal-Muñoz; Francisco Ramos-Morales
Journal:  J Bacteriol       Date:  2013-03-15       Impact factor: 3.490

6.  The temperature sensitive mutant 72c. I. Pleiotropic growth behaviour and changed response to some antibiotics and mutations in the transcription or translation apparatus.

Authors:  L A Isaksson; R Takata
Journal:  Mol Gen Genet       Date:  1978-04-25

7.  The temperature sensitive mutant 72c. II. Accumulation at high temperature of ppGpp and pppGpp in the presence of protein synthesis.

Authors:  R Takata; L A Isaksson
Journal:  Mol Gen Genet       Date:  1978-04-25

8.  Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth.

Authors:  K Bohman; L A Isaksson
Journal:  Mol Gen Genet       Date:  1978-05-31

9.  Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica.

Authors:  Eva M Camacho; Ana Serna; Cristina Madrid; Silvia Marqués; Raúl Fernández; Fernando de la Cruz; Antonio Juárez; Josep Casadesús
Journal:  J Bacteriol       Date:  2005-08       Impact factor: 3.490

10.  Nucleoside triphosphate regeneration decreases the frequency of translation errors.

Authors:  P C Jelenc; C G Kurland
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

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