Literature DB >> 4903811

Preparation of isolated rat liver mitochondria for electron microscopy.

W H Butler, J D Judah.   

Abstract

A comparative study of the fixation of isolated rat liver mitochondria was undertaken. If the criterion is adopted that after processing, the mitochondria should resemble as closely as possible rat liver mitochondria in situ, the procedure found to produce such preservation was that of fixation in suspension in veronal-buffered 2% potassium permanganate. Fixation in osmium tetroxide produced variable results, while mitochondria fixed in glutaraldehyde were contracted. We suggest that in cases where fixation procedures modify the morphological appearance of mitochondria, the significance of such changes must be treated with caution.

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Year:  1970        PMID： 4903811      PMCID： PMC2107945          DOI： 10.1083/jcb.44.2.278

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  18 in total

1.  The fine structure of mitochondria.

Authors:  G E PALADE
Journal:  Anat Rec       Date:  1952-11

2.  Morphological variations of isolated liver mitochondria during spontaneous deterioration.

Authors:  M T O'Hegarty; J W Harman
Journal:  Z Zellforsch Mikrosk Anat       Date:  1966

3.  Effect of modifications in conformation of protein molecules on structure of mitochondrial membranes.

Authors:  F S Sjöstrand; L Barajas
Journal:  J Ultrastruct Res       Date:  1968-10

4.  Glutaraldehyde as a protein cross-linkage reagent.

Authors:  F M Richards; J R Knowles
Journal:  J Mol Biol       Date:  1968-10-14       Impact factor: 5.469

5.  Ultrastructural bases for metabolically linked mechanical activity in mitochondria. I. Reversible ultrastructural changes with change in metabolic steady state in isolated liver mitochondria.

Authors:  C R Hackenbrock
Journal:  J Cell Biol       Date:  1966-08       Impact factor: 10.539

6.  Oscillatory states of mitochondria. 3. Ultrastructure of trapped conformational states.

Authors:  D W Deamer; K Utsumi; L Packer
Journal:  Arch Biochem Biophys       Date:  1967-09       Impact factor: 4.013

7.  STRUCTURAL CHANGES IN ISOLATED LIVER MITOCHONDRIA OF RATS DURING ESSENTIAL FATTY ACID DEFICIENCY.

Authors:  J A SMITH; H F DELUCA
Journal:  J Cell Biol       Date:  1964-04       Impact factor: 10.539

8.  Improvements in epoxy resin embedding methods.

Authors:  J H LUFT
Journal:  J Biophys Biochem Cytol       Date:  1961-02

9.  Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.

Authors:  J Lenard; S J Singer
Journal:  J Cell Biol       Date:  1968-04       Impact factor: 10.539

10.  Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.

Authors:  D D SABATINI; K BENSCH; R J BARRNETT
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539
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  4 in total

1.  Ultrastructural studies on mitochondrial swelling.

Authors:  W H Butler; J D Judah
Journal:  Biochem J       Date:  1970-08       Impact factor: 3.857

2.  On the "energized-twisted" configuration of isolated beef heart mitochondria.

Authors:  G R Hunter; G P Brierley
Journal:  J Cell Biol       Date:  1971-07       Impact factor: 10.539

3.  Energy-linked ultrastructural transformations in isolated liver mitochondria and mitoplasts. Preservation of configurations by freeze-cleaving compared to chemical fixation.

Authors:  C R Hackenbrock
Journal:  J Cell Biol       Date:  1972-05       Impact factor: 10.539

4.  A study of rapid mitochondrial structural changes in vitro by spray-freeze-etching.

Authors:  R D Lang; J R Bronk
Journal:  J Cell Biol       Date:  1978-04       Impact factor: 10.539
  4 in total

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