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Literature DB >> 5645836

Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.

Abstract

The effects of several commonly employed fixatives on the three-dimensional conformations of two soluble proteins and the protein of intact red blood cell membranes have been studied by means of circular dichroism measurements in the spectral region of the peptide absorption bands. The fixatives used produced significant and parallel conformational changes in all of the proteins, in the increasing order: glutaraldehyde; OsO(4); glutaraldehyde followed by OsO(4); and KMnO(4). The last two treatments obliterated most of the helical character of the proteins. The significance of these observations to the preparation of specimens for electron microscopy is discussed.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1968 PMID： 5645836 PMCID： PMC2107400 DOI： 10.1083/jcb.37.1.117

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

15 in total

Review 1. THE DISSOCIATION AND ASSOCIATION OF PROTEIN STRUCTURES.

Authors: F J REITHEL
Journal: Adv Protein Chem Date: 1963

2. RELATIVE CONFORMATIONS OF SPERM WHALE METMYOGLOBIN AND APOMYOGLOBIN IN SOLUTION.

Authors: E BRESLOW; S BEYCHOK; K D HARDMAN; F R GURD
Journal: J Biol Chem Date: 1965-01 Impact factor: 5.157

3. THE EFFECT OF THE FIXATIVE SOLUTION ON THE ULTRASTRUCTURE OF CELLS AND TISSUES. A COMPARATIVE ANALYSIS WITH PARTICULAR ATTENTION TO THE PROXIMAL CONVOLUTED TUBULE OF THE RAT KIDNEY.

Authors: B F TRUMP; J L ERICSSON
Journal: Lab Invest Date: 1965-06 Impact factor: 5.662

4. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.

Authors: J T DODGE; C MITCHELL; D J HANAHAN
Journal: Arch Biochem Biophys Date: 1963-01 Impact factor: 4.013

5. Cleavage of the haem-protein link by acid methylethylketone.

Authors: F W TEALE
Journal: Biochim Biophys Acta Date: 1959-10

6. The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives.

Authors: A B Maunsbach
Journal: J Ultrastruct Res Date: 1966-06

7. Optical rotation of mitochondrial membranes.

Authors: D W Urry; M Mednieks; E Bejnarowicz
Journal: Proc Natl Acad Sci U S A Date: 1967-04 Impact factor: 11.205

8. Protein conformations in cellular membranes.

Authors: D F Wallach; P H Zahler
Journal: Proc Natl Acad Sci U S A Date: 1966-11 Impact factor: 11.205

9. Glutaraldehyde activation of nuclear acid phosphatase in cultured plant cells.

Authors: D W De Jong; A C Olson; E F Jansen
Journal: Science Date: 1967-03-31 Impact factor: 47.728

Review 10. Structure of biological membranes.

Authors: E D Korn
Journal: Science Date: 1966-09-23 Impact factor: 47.728

47 in total

5. A study of the ultrastructural organization of cytochrome c-phospholipid membranes as revealed by various experimental treatments.

Authors: V L Borovjagin; D A Moshkov
Journal: J Membr Biol Date: 1973-10-10 Impact factor: 1.843

10. Influence of glutaraldehyde and-or osmium tetroxide on cell volume, ion content, mechanical stability, and membrane permeability of Ehrlich ascites tumor cells.

Authors: A Penttila; H Kalimo; B F Trump
Journal: J Cell Biol Date: 1974-10 Impact factor: 10.539

Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.

Review 1. THE DISSOCIATION AND ASSOCIATION OF PROTEIN STRUCTURES.

2. RELATIVE CONFORMATIONS OF SPERM WHALE METMYOGLOBIN AND APOMYOGLOBIN IN SOLUTION.

3. THE EFFECT OF THE FIXATIVE SOLUTION ON THE ULTRASTRUCTURE OF CELLS AND TISSUES. A COMPARATIVE ANALYSIS WITH PARTICULAR ATTENTION TO THE PROXIMAL CONVOLUTED TUBULE OF THE RAT KIDNEY.

4. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.

5. Cleavage of the haem-protein link by acid methylethylketone.

6. The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives.

7. Optical rotation of mitochondrial membranes.

8. Protein conformations in cellular membranes.

9. Glutaraldehyde activation of nuclear acid phosphatase in cultured plant cells.

Review 10. Structure of biological membranes.

1. Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.

2. Electron tomographic analysis of synaptic ultrastructure.

3. Subpial glial limiting membrane of the cat spinal cord visualized by scanning electron microscopy.

4. Interaction forces between red cells agglutinated by antibody. II. Measurement of hydrodynamic force of breakup.

5. A study of the ultrastructural organization of cytochrome c-phospholipid membranes as revealed by various experimental treatments.

6. Biochemical aspects of aldehyde fixation and a new formaldehyde fixative.

7. The mechanisms of glutaraldehyde-fixed sarcoma 180 ascites cell aggregation.

8. Myoepithelial cells in human thymus: staining, polarization and fluorescence microscopic studies.

9. Preservation of erythrocyte ghost ultrastructure achieved by various fixatives.

10. Influence of glutaraldehyde and-or osmium tetroxide on cell volume, ion content, mechanical stability, and membrane permeability of Ehrlich ascites tumor cells.