Literature DB >> 486122

The rapid preparation of peroxisomes from rat liver by using a vertical rotor.

C E Neat, H Osmundsen.   

Abstract

A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.

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Year:  1979        PMID： 486122      PMCID： PMC1161073          DOI： 10.1042/bj1800445

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  ISOLATION OF RAT-LIVER LYSOSOMES AND THEIR GENERAL PROPERTIES.

Authors:  P L SAWANT; U S KUMTA; A L TAPPEL
Journal:  Biochim Biophys Acta       Date:  1964-04-06

2.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors:  C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal:  Biochem J       Date:  1955-08       Impact factor: 3.857

3.  Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue.

Authors:  P Baudhuin; H Beaufay; Y Rahman-Li; O Z Sellinger; R Wattiaux; P Jacques; C De Duve
Journal:  Biochem J       Date:  1964-07       Impact factor: 3.857

4.  A convenient and versatile method for the purification of CoA thiol esters.

Authors:  M E Pullman
Journal:  Anal Biochem       Date:  1973-07       Impact factor: 3.365

5.  Isolation of rat liver peroxisomes.

Authors:  P Baudhuin
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  Comparison of fatty acid oxidation in mitochondria and peroxisomes from rat liver.

Authors:  J B Krahling; R Gee; P A Murphy; J R Kirk; N E Tolbert
Journal:  Biochem Biophys Res Commun       Date:  1978-05-15       Impact factor: 3.575

7.  A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug.

Authors:  P B Lazarow; C De Duve
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

8.  Localization of enzymes within microbodies.

Authors:  A H Huang; H Beevers
Journal:  J Cell Biol       Date:  1973-08       Impact factor: 10.539

9.  Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study.

Authors:  A Blouin; R P Bolender; E R Weibel
Journal:  J Cell Biol       Date:  1977-02       Impact factor: 10.539

10.  The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.

Authors:  F Leighton; B Poole; H Beaufay; P Baudhuin; J W Coffey; S Fowler; C De Duve
Journal:  J Cell Biol       Date:  1968-05       Impact factor: 10.539
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  11 in total

1.  Distribution of Fhit protein in rat tissues and its intracellular localization.

Authors:  F Golebiowski; R Kowara; T Pawelczyk
Journal:  Mol Cell Biochem       Date:  2001-10       Impact factor: 3.396

2.  A human autoantibody to peroxisomes.

Authors:  R Holm; P I Gaarder; L Helgeland; E I Falkenhaug
Journal:  Clin Exp Immunol       Date:  1985-08       Impact factor: 4.330

3.  Hepatic peroxisomal and mitochondrial fatty acid oxidation in the riboflavin-deficient rat.

Authors:  P S Brady; C L Hoppel
Journal:  Biochem J       Date:  1985-08-01       Impact factor: 3.857

4.  Elevated hepatic mitochondrial and peroxisomal oxidative capacities in fed and starved adult obese (ob/ob) mice.

Authors:  L J Brady; P S Brady; D R Romsos; C L Hoppel
Journal:  Biochem J       Date:  1985-10-15       Impact factor: 3.857

5.  Induction of peroxisomal beta-oxidation in rat liver by high-fat diets.

Authors:  C E Neat; M S Thomassen; H Osmundsen
Journal:  Biochem J       Date:  1980-01-15       Impact factor: 3.857

6.  Induction, immunochemical identity and immunofluorescence localization of an 80 000-molecular-weight peroxisome-proliferation-associated polypeptide (polypeptide PPA-80) and peroxisomal enoyl-CoA hydratase of mouse liver and renal cortex.

Authors:  N D Lalwani; M K Reddy; M Mangkornkanok-Mark; J K Reddy
Journal:  Biochem J       Date:  1981-07-15       Impact factor: 3.857

7.  Influence of partially hydrogenated vegetable and marine oils on lipid metabolism in rat liver and heart.

Authors:  G Hølmer; C E Høy; D Kirstein
Journal:  Lipids       Date:  1982-09       Impact factor: 1.880

8.  Effects of high-fat diets on hepatic fatty acid oxidation in the rat. Isolation of rat liver peroxisomes by vertical-rotor centrifugation by using a self-generated, iso-osmotic, Percoll gradient.

Authors:  C E Neat; M S Thomassen; H Osmundsen
Journal:  Biochem J       Date:  1981-04-15       Impact factor: 3.857

9.  Occurrence of a long-chain delta 3,delta 2-enoyl-CoA isomerase in rat liver.

Authors:  J M Kilponen; P M Palosaari; J K Hiltunen
Journal:  Biochem J       Date:  1990-07-01       Impact factor: 3.857

10.  Partial disassembly of peroxisomes.

Authors:  S E Alexson; Y Fujiki; H Shio; P B Lazarow
Journal:  J Cell Biol       Date:  1985-07       Impact factor: 10.539
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