Literature DB >> 486121

The separation of microsomal membranes and free polyribosomes by gel filtration.

N McCole, D N Palmer, D J Williams.   

Abstract

The integrity of polyribosome-membrane complexes in microsomal preparations is dependent on the centrifugal conditions used for their preparation. This paper describes techniques based on gel filtration that enable the rapid and gentle separation of microsomal membranes from both free polyribosomes and soluble protein.

Mesh:

Year:  1979        PMID: 486121      PMCID: PMC1161071          DOI: 10.1042/bj1800437

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  4 in total

1.  Isolation of rat liver microsomes by gel filtration.

Authors:  O Tangen; J Jonsson; S Orrenius
Journal:  Anal Biochem       Date:  1973-08       Impact factor: 3.365

2.  Use of gel filtration to monitor ribosome-membrane interaction.

Authors:  A L Ibbetson; R B Freedman
Journal:  Biochem Soc Trans       Date:  1975       Impact factor: 5.407

3.  Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration.

Authors:  M García-Patrone; C A Perazzolo; I D Algranati
Journal:  Acta Physiol Lat Am       Date:  1976

4.  A sub-population of rat liver membrane-bound ribosomes that are detached in vitro by carcinogens and centrifugation.

Authors:  D N Palmer; B R Rabin; D J Williams
Journal:  Biochem J       Date:  1978-10-15       Impact factor: 3.857

  4 in total
  1 in total

1.  Preparation and properties of a complex from rat liver of polyribosomes with components of the cytoskeleton.

Authors:  A Adams; E G Fey; S F Pike; C J Taylorson; H A White; B R Rabin
Journal:  Biochem J       Date:  1983-10-15       Impact factor: 3.857

  1 in total

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