Literature DB >> 4742564

New colorimetric method for the quantitative estimation of phospholipids without acid digestion.

R K Raheja, C Kaur, A Singh, I S Bhatia.   

Abstract

A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res., 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1-10 micro g of phospholipid phosphorus.

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Year:  1973        PMID: 4742564

Source DB:  PubMed          Journal:  J Lipid Res        ISSN: 0022-2275            Impact factor:   5.922


  60 in total

1.  Relative concentrations of 14C-DDT and of two polychlorinated biphenyls in the lipids of cod tissues after a single oral dose.

Authors:  A I Mitchell; P A Plack; I M Thomson
Journal:  Arch Environ Contam Toxicol       Date:  1977       Impact factor: 2.804

2.  Microencapsulation of solid dispersions: release of griseofulvin from griseofulvin:phospholipid coprecipitates in microspheres.

Authors:  G K Vudathala; J A Rogers
Journal:  Pharm Res       Date:  1992-06       Impact factor: 4.200

3.  Lipid order and composition of synaptic membranes in experimental diabetes mellitus.

Authors:  A D Mooradian; F Dickerson; T L Smith
Journal:  Neurochem Res       Date:  1990-10       Impact factor: 3.996

4.  Fish diet and physical fitness in relation to membrane and serum lipids, prostanoid metabolism and platelet aggregation in female students.

Authors:  J J Agren; H Pekkarinen; H Litmanen; O Hänninen
Journal:  Eur J Appl Physiol Occup Physiol       Date:  1991

5.  Desiccation-induced ROS accumulation and lipid catabolism in recalcitrant Madhuca latifolia seeds.

Authors:  Jipsi Chandra; S Keshavkant
Journal:  Physiol Mol Biol Plants       Date:  2017-12-18

6.  Fatty acid metabolism in L1210 murine leukemia cells: differences in modification of fatty acids incorporated into various lipids.

Authors:  C P Burns; S P Wei; A A Spector
Journal:  Lipids       Date:  1978-10       Impact factor: 1.880

7.  Studies of the thermotropic phase behavior of phosphatidylcholines containing 2-alkyl substituted fatty acyl chains: a new class of phosphatidylcholines forming inverted nonlamellar phases.

Authors:  R N Lewis; R N McElhaney; P E Harper; D C Turner; S M Gruner
Journal:  Biophys J       Date:  1994-04       Impact factor: 4.033

8.  Kidney lipids: changes caused by dietary 9-trans, 12-trans-octadecadienoate.

Authors:  J E Kinsella; P H Yu; J B Mai
Journal:  Lipids       Date:  1979-12       Impact factor: 1.880

9.  Comparison of the metabolism of chylomicrons and chylomicron remnants by the perfused liver.

Authors:  R S Gardner; P A Mayes
Journal:  Biochem J       Date:  1978-01-15       Impact factor: 3.857

10.  Prostaglandins and their precursors in tissues from rats fed on trans, trans-linoleate.

Authors:  J E Kinsella; D H Hwang; P Yu; J Mai; J Shimp
Journal:  Biochem J       Date:  1979-12-15       Impact factor: 3.857

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