| Literature DB >> 4742564 |
R K Raheja, C Kaur, A Singh, I S Bhatia.
Abstract
A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res., 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1-10 micro g of phospholipid phosphorus.Entities:
Mesh:
Substances:
Year: 1973 PMID: 4742564
Source DB: PubMed Journal: J Lipid Res ISSN: 0022-2275 Impact factor: 5.922