Literature DB >> 4673020

Optimization of the interferon assay using inhibition of Semliki forest virus-ribonucleic acid synthesis.

H Koblet, U Kohler, R Wyler.   

Abstract

The method for assaying chicken interferon by its inhibition of viral ribonucleic acid (RNA) synthesis was optimized for the chicken embryo fibroblast-Semliki Forest virus (SFV) system, with respect to time, multiplicity of infection, and addition of actinomycin D and (3)H-uridine. Incorporation of (3)H into viral RNA is reproducible, and amounts to 10(4) to 2 x 10(4) counts per min per uninhibited culture per 1 muCi per 6 hr. The assay may be carried out in less than 1 day and is sensitive (0.05 units/ml) and exact (+/-20% of the mean titers on different days); it can be used for purification procedures as well.

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Year:  1972        PMID： 4673020      PMCID： PMC376518          DOI： 10.1128/am.24.3.323-327.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  5 in total

1.  QUANTITATIVE HAEMADSORPTION, A NEW ASSAY TECHNIQUE. I. ASSAY OF INTERFERON.

Authors:  N B FINTER
Journal:  Virology       Date:  1964-12       Impact factor: 3.616

2.  Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon.

Authors:  J LINDENMANN; G E GIFFORD
Journal:  Virology       Date:  1963-03       Impact factor: 3.616

3.  Assay of chick interferons by the inhibition of viral ribonucleic acid synthesis.

Authors:  M McWilliams; M S Finkelstein; P T Allen; D J Giron
Journal:  Appl Microbiol       Date:  1971-05

4.  Rapid sensitive assay for interferons based on the inhibition of MM virus nucleic acid synthesis.

Authors:  P T Allen; D J Giron
Journal:  Appl Microbiol       Date:  1970-09

5.  Replication of Venezuelan equine encephalomyelitis virus in vitro. II. Viral growth response to selected nutritional additives in suspension cultures.

Authors:  H J Hearn; H R Tribble; O C Bowersox; S C Nagle
Journal:  Appl Microbiol       Date:  1971-02
  5 in total
  9 in total

1.  The enhancing effect of copper, nickel, and cobalt ions on plaque formation by Semliki Forest virus (SFV) in chicken embryo fibroblasts.

Authors:  R Wyler; W Wiesendanger
Journal:  Arch Virol       Date:  1975       Impact factor: 2.574

2.  Microassay for interferon, using [3H]uridine, microculture plates, and a multiple automated sample harvester.

Authors:  J Y Richmond; J Polatnick; R C Knudsen
Journal:  Appl Environ Microbiol       Date:  1980-04       Impact factor: 4.792

3.  An imidazole derivative (Econazole) as an antifungal agent in cell culture systems.

Authors:  R Wyler; A Murbach; H Möhl
Journal:  In Vitro       Date:  1979-10

4.  The effect of cordycepin on the multiplication of Semliki Forest virus and on polyadenylation of viral RNA.

Authors:  R Wittek; H Koblet; A Menna; R Wyler
Journal:  Arch Virol       Date:  1977       Impact factor: 2.574

5.  Kinetics of interferon action.

Authors:  H Koblet; R Wyler; U Kohler
Journal:  Experientia       Date:  1978-09-15

6.  Increase of interferon antiviral activity by exogenous cyclic adenosine-3':5'-monophosphate (cAMP).

Authors:  H Koblet; R Wyler; U Kohler
Journal:  Experientia       Date:  1979-05-15

7.  Actinomycin-resistant antiviral activity associated with preparations containing chicken interferon.

Authors:  P Dossenbach; H Koblet; R Wyler
Journal:  Experientia       Date:  1976-12-15

8.  Interferon system in patients with systemic juvenile chronic arthritis: in vivo and in vitro studies.

Authors:  T H Bacon; A de Vere-Tyndall; D A Tyrrell; A M Denman; B M Ansell
Journal:  Clin Exp Immunol       Date:  1983-10       Impact factor: 4.330

9.  Altered or increased transfer-RNA methylation in the course of Interferon action on cells in culture?

Authors:  H Koblet; R Wyler; U Kohler
Journal:  Experientia       Date:  1979-05-15
  9 in total

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