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Literature DB >> 4656791

Light-scattering studies on deoxyribonucleic acid flexibility. The solution properties of a small circular deoxyribonucleic acid molecule.

Abstract

Previous investigations on the persistence length of DNA in solution have revealed large discrepancies between hydrodynamic results and those from light-scattering techniques which have potentially a greater resolving power. The information obtained from experiments on a small circular DNA molecule has resolved these discrepancies. The non-superhelical circular double-stranded DNA molecule from bacteriophage [unk]X174-infected cells is small enough to permit accurate light-scattering extrapolations, and its solutions have negligible anisotropy. The persistence length obtained from experimental investigations on this molecule is comparable with that obtained by hydrodynamic techniques, even with variation of the excluded-volume factor.

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Year: 1972 PMID： 4656791 PMCID： PMC1174552 DOI： 10.1042/bj1301019

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

19 in total

4. Deoxyribonucleate solutions: sedimentation in a density gradient, partial specific volumes, density and refractive index increments, and preferential interactions.

Authors: G Cohen; H Eisenberg
Journal: Biopolymers Date: 1968 Impact factor: 2.505

3. Aspects of specific protein-DNA interaction; multi-mode binding of the oligopeptide antibiotic netropsin to (A.T)-rich DNA segments.

Authors: K E Reinert; E Stutter; H Schweiss
Journal: Nucleic Acids Res Date: 1979-11-10 Impact factor: 16.971

4. Light-scattering studies on supercoil unwinding.

Authors: A M Campbell; D J Jolly
Journal: Biochem J Date: 1973-06 Impact factor: 3.857

4 in total

Light-scattering studies on deoxyribonucleic acid flexibility. The solution properties of a small circular deoxyribonucleic acid molecule.

1. Physicochemical studies on the minicircular DNA in Escherichia coli 15.

2. Low-angle light-scattering instrument for DNA solutions.

3. Intrinsic viscosity of wormlike chains with excluded-volume effects.

4. Deoxyribonucleate solutions: sedimentation in a density gradient, partial specific volumes, density and refractive index increments, and preferential interactions.

5. Light scattering from wormlike chains with excluded volume effects.

6. Low-angle light-scattering studies on alkali- and heat-denatured DNA.

7. Electro-optic scattering studies on deoxyribonucleic acid.

8. Intrinsic viscosity of wormlike polymer chains.

9. Molecular weights of homogeneous coliphage DNA's from density-gradient sedimentation equilibrium.

10. Conformation studies on the sodium and cesium salts of calf thymus deoxyribonucleic acid (DNA).

1. The effects of deoxyribonucleic acid secondary structure on tertiary structure.

2. Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. The effect of size on supercoil shape.

3. Aspects of specific protein-DNA interaction; multi-mode binding of the oligopeptide antibiotic netropsin to (A.T)-rich DNA segments.

4. Light-scattering studies on supercoil unwinding.