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Literature DB >> 938468

Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. The effect of size on supercoil shape.

Abstract

Laser light-scattering studies of bacteriophage PM2 DNA showed the molecule to have mol.wt. 5.9 X 10(6) and root-mean -square radius 125 nm at an ionic strength of 0.2 mol/litre. Computer-generated curves compatible with these data were compared with the experimental interference curve for several structural models of the molecules. The data fit best to an asymmetric four-armed planar molecule in which all four arms emerge from or close to the one area of the molecule. This contrasts with the smaller DNA molecules investigated, which have shown a three-armed molecule, whose symmetry varies with primary structure.

Entities: Species

Mesh：

Substances：
DNA, Viral

Year: 1976 PMID： 938468 PMCID： PMC1172806 DOI： 10.1042/bj1550101

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

20 in total

1. The sense of naturally occurring superhelices and the unwinding angle of intercalated ethidium.

Authors: D E Pulleyblank; A R Morgan
Journal: J Mol Biol Date: 1975-01-05 Impact factor: 5.469

2. Denaturation of polyoma DNA by phage T4 gene 32 protein.

Authors: J Monjardino; A W James
Journal: Nature Date: 1975-05-15 Impact factor: 49.962

3. Effects of DNA primary structure on tertiary structure.

Authors: A Campbell; R Eason
Journal: FEBS Lett Date: 1975-07-15 Impact factor: 4.124

4. Effect of circularity and superhelicity on transcription from bacteriophagelambda DNA.

Authors: P Botchan; J C Wang; H Echols
Journal: Proc Natl Acad Sci U S A Date: 1973-11 Impact factor: 11.205

5. Characterization of the single-strand-specific nuclease S1 activity on double-stranded supercoiled polyoma DNA.

Authors: J E Germond; V M Vogt; B Hirt
Journal: Eur J Biochem Date: 1974-04-16

6. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

Authors: J C Wang
Journal: J Mol Biol Date: 1974-11-15 Impact factor: 5.469

7. The structure of psi DNA.

Authors: T Maniatis; J H Venable; L S Lerman
Journal: J Mol Biol Date: 1974-03-25 Impact factor: 5.469

8. Location of the T4 gene 32 protein binding site on simian virus 40 DNA.

Authors: J F Morrow; P Berg
Journal: J Virol Date: 1973-12 Impact factor: 5.103

9. Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease.

Authors: P Beard; J F Morrow; P Berg
Journal: J Virol Date: 1973-12 Impact factor: 5.103

10. The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.

Authors: B Hudson; W B Upholt; J Devinny; J Vinograd
Journal: Proc Natl Acad Sci U S A Date: 1969-03 Impact factor: 11.205

9 in total

Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. The effect of size on supercoil shape.

1. The sense of naturally occurring superhelices and the unwinding angle of intercalated ethidium.

2. Denaturation of polyoma DNA by phage T4 gene 32 protein.

3. Effects of DNA primary structure on tertiary structure.

4. Effect of circularity and superhelicity on transcription from bacteriophagelambda DNA.

5. Characterization of the single-strand-specific nuclease S1 activity on double-stranded supercoiled polyoma DNA.

6. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

7. The structure of psi DNA.

8. Location of the T4 gene 32 protein binding site on simian virus 40 DNA.

9. Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease.

10. The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.

1. Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives.

2. The effects of deoxyribonucleic acid secondary structure on tertiary structure.

3. Superstructure of linear duplex DNA.

4. The interaction of RNA polymerase II from wheat with supercoiled and linear plasmid templates.

5. The dissociation of sigma-factor from ribonucleic acid polymerase.

6. Conformational variation in superhelical deoxyribonucleic acid.

7. X-ray scattering from randomly oriented superhelices. Circular superhelical DNA.

8. Light scattering measurements supporting helical structures for chromatin in solution.

9. Subunit associations among chromatin particles.