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Literature DB >> 4631666

( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis.

Abstract

DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [(6)N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65-0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA).

Entities: Chemical Species

Mesh：

Substances：
Tritium
DNA
Adenine

Year: 1973 PMID： 4631666 PMCID： PMC2108936 DOI： 10.1083/jcb.56.3.697

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

21 in total

( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis.

1. Inhibition of modification and restriction for phages lambda and T-1 by co-infecting T3.

2. [Regulation of the amount of DNA in the macronucleus of Tetrahymena].

3. The purification and properties of deoxyribonucleic acid methylase from rat spleen.

4. Methylated bases in DNA of animal origin.

5. [Mechanisms of the host-controlled modification of phage T1].

6. Deoxyribonucleic acid methylase activity in pea seedlings.

7. The in vivo methylation of DNA in mouse fibroblasts.

8. Deoxyribonucleic acid methylase of mammalian tissues.

9. Methylation of nucleic acids in Krebs II ascites tumour cells.

10. The fine structure of the nuclei of Tetrahymena pyriformis throughout the cell cycle.

1. Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1.

2. Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila.

3. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

4. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

5. Comparative study of DNA methylation in three unicellular eucaryotes.

6. Stage-specific DNA methylation in a fungal plant pathogen.

7. Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.

8. Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila.

9. 5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.

10. Sequence organization within and flanking clusters of 5S ribosomal RNA genes in Tetrahymena.