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Literature DB >> 4628873

In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I.

Abstract

DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.

Entities: Disease Species

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Year: 1972 PMID： 4628873 PMCID： PMC389738 DOI： 10.1073/pnas.69.11.3211

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors: K BURTON
Journal: Biochem J Date: 1956-02 Impact factor: 3.857

2. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors: C Anagnostopoulos; J Spizizen
Journal: J Bacteriol Date: 1961-05 Impact factor: 3.490

3. The deoxyribo-nucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid.

Authors: I R LEHMAN; G G ROUSSOS; E A PRATT
Journal: J Biol Chem Date: 1962-03 Impact factor: 5.157

4. Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli.

Authors: P K Cooper; P C Hanawalt
Journal: Proc Natl Acad Sci U S A Date: 1972-05 Impact factor: 11.205

5. Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells.

Authors: M Monk; M Peacey; J D Gross
Journal: J Mol Biol Date: 1971-06-14 Impact factor: 5.469

6. Repair deficiency in a bacterial mutant defective in DNA polymerase.

Authors: L Kanner; P Hanawalt
Journal: Biochem Biophys Res Commun Date: 1970-04-08 Impact factor: 3.575

7. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

Authors: T M Jovin; P T Englund; L L Bertsch
Journal: J Biol Chem Date: 1969-06-10 Impact factor: 5.157

8. Resistance of DNA against radiation-induced strand breakage in bacterial spores.

Authors: H Tanooka; H Terano
Journal: Radiat Res Date: 1970-09 Impact factor: 2.841

9. Repair of radiation-induced damage in Escherichia coli. II. Effect of rec and uvr mutations on radiosensitivity, and repair of x-ray-induced single-strand breaks in deoxyribonucleic acid.

Authors: D S Kapp; K C Smith
Journal: J Bacteriol Date: 1970-07 Impact factor: 3.490

In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I.

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

2. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

3. The deoxyribo-nucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid.

4. Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli.

5. Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells.

6. Repair deficiency in a bacterial mutant defective in DNA polymerase.

7. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

8. Resistance of DNA against radiation-induced strand breakage in bacterial spores.

9. Repair of radiation-induced damage in Escherichia coli. II. Effect of rec and uvr mutations on radiosensitivity, and repair of x-ray-induced single-strand breaks in deoxyribonucleic acid.

10. Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation.

1. In vitro repair of UV-or x-irradiated bacteriophage T4 DNA by extract from blue-green alga Anacystis nidulans.

2. Enzymic in vitro repair and chemical nature of DNA chain breaks induced by incorporated phosphorus-32P decay.

3. Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.

4. Dying of gamma-irradiated Escherichia coli studied by the use of prophage.

5. The involvement of polynucleotide ligase in the repair of UV-induced DNA damage in Escherichia coli K-12 cells.

6. In vitro packaging of UV radiation-damaged DNA from bacteriophage T7.

7. Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation.