| Literature DB >> 4623279 |
D G Saymen, P Nathan, I A Holder, E O Hill, B G Macmillan.
Abstract
Methods for the quantitation of bacteria in infected tissues must be rigidly standardized to insure uniformity of results. In this communication we report on a laboratory animal model for the study of surface wound infection and the development of a standardized method for the quantitative estimation of bacteria in infected surface wound tissue by mechanical tissue homogenization and serial dilution. Parallel comparative studies demonstrated that a moist-swab sampling procedure detected only 10% of the bacteria recoverable by a surface-wash procedure. Either tissue homogenization or surface-wash procedures recovered significantly more bacteria from contaminated surface wounds than were obtained by surface-swab sampling techniques.Entities:
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Year: 1972 PMID: 4623279 PMCID: PMC380379 DOI: 10.1128/am.23.3.509-514.1972
Source DB: PubMed Journal: Appl Microbiol ISSN: 0003-6919