Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Differentiation of neuromuscular junctions in cultures of embryonic Drosophila cells.

Literature DB >> 4622290

Differentiation of neuromuscular junctions in cultures of embryonic Drosophila cells.

R L Seecof, R L Teplitz, I Gerson, K Ikeda, J Donady.

Abstract

Cultures were prepared of embryonic cells from Drosophila melanogaster. Neurons and myocytes differentiated in vitro from their respective stem cells. Electron microscopy showed that neuromuscular junctions formed where axons contacted myocytes. Electrical stimuli were applied to axons and these caused contractions of innervated myocytes. This is the first report of insect or other invertebrate neuromuscular junctions differentiating in vitro. In addition, this is the first system reported in which the neurons, myocytes, and junctions are completely differentiated in vitro from neuroblasts and myoblasts.

Entities: Disease Species

Mesh：

Year: 1972 PMID： 4622290 PMCID： PMC426508 DOI： 10.1073/pnas.69.3.566

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

8 in total

1. Differentiation of neurons and myocytes in cell cultures made from Drosophila gastrulae.

Authors: R L Seecof; N Alléaume; R L Teplitz; I Gerson
Journal: Exp Cell Res Date: 1971-11 Impact factor: 3.905

2. Developing neuromuscular junctions: first signs of chemical transmission during formation in tissue culture.

Authors: N Robbins; T Yonezawa
Journal: Science Date: 1971-04-23 Impact factor: 47.728

3. Differentiation of embryonic Drosophila cells in vitro.

Authors: R L Seecof; R L Unanue
Journal: Exp Cell Res Date: 1968-06 Impact factor: 3.905

4. De novo formation of neuro-muscular junctions in tissue culture.

Authors: D W James; R L Tresman
Journal: Nature Date: 1968-10-26 Impact factor: 49.962

5. Bioelectric interactions between cultured fetal rodent spinal cord and skeletal muscle after innervation in vitro.

Authors: S M Crain
Journal: J Exp Zool Date: 1970-04

6. Synaptic potentials recorded in cell cultures of nerve and muscle.

Authors: G D Fischbach
Journal: Science Date: 1970-09-25 Impact factor: 47.728

7. Formation de novo and development of neuromuscular junctions in vitro.

Authors: G Veneroni; M R Murray
Journal: J Embryol Exp Morphol Date: 1969-04

8. Formation of neuromuscular junctions in embryonic cell cultures.

Authors: Y Shimada; D A Fischman; A A Moscona
Journal: Proc Natl Acad Sci U S A Date: 1969-03 Impact factor: 11.205

8 in total

15 in total

10. Single cell cultures of Drosophila neuroectodermal and mesectodermal central nervous system progenitors reveal different degrees of developmental autonomy.

Authors: Karin Lüer; Gerhard M Technau
Journal: Neural Dev Date: 2009-08-03 Impact factor: 3.842

Differentiation of neuromuscular junctions in cultures of embryonic Drosophila cells.

1. Differentiation of neurons and myocytes in cell cultures made from Drosophila gastrulae.

2. Developing neuromuscular junctions: first signs of chemical transmission during formation in tissue culture.

3. Differentiation of embryonic Drosophila cells in vitro.

4. De novo formation of neuro-muscular junctions in tissue culture.

5. Bioelectric interactions between cultured fetal rodent spinal cord and skeletal muscle after innervation in vitro.

6. Synaptic potentials recorded in cell cultures of nerve and muscle.

7. Formation de novo and development of neuromuscular junctions in vitro.

8. Formation of neuromuscular junctions in embryonic cell cultures.

1. Synaptoid profiles in regenerating crustacean peripheral nerves.

2. Silencing of genes in cultured Drosophila neurons by RNA interference.

3. Characterization of early steps in muscle morphogenesis in a Drosophila primary culture system.

4. Differentiation in vitro of larval and adult muscles from embryonic cells ofDrosophila.

5. Synaptic differentiation in a regenerating crab-limb muscle.

6. Effect of the gene lethal (1) myospheroid on Drosophila embryonic cells in vitro.

7. Ultrastructural differentiation during embryonic Drosophila myogenesis in vitro.

8. In vitro imaging of primary neural cell culture from Drosophila.

9. Teratogens induce a subset of small heat shock proteins in Drosophila primary embryonic cell cultures.

10. Single cell cultures of Drosophila neuroectodermal and mesectodermal central nervous system progenitors reveal different degrees of developmental autonomy.