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Literature DB >> 4618777

A mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from Klebsiella aerogenes.

H R Morris, D H Williams, G G Midwinter, B S Hartley.

Abstract

The first detailed results of the application of a low-resolution mixture analysis approach to the sequence analysis of an enzyme, ribitol dehydrogenase, are given. Examples of the interpretation of the spectra of peptide mixtures derived from this protein are described. Evidence for new fragmentation patterns observed is reported, together with an explanation of the generation of ambiguous sequences by use of a low-specificity enzyme, thermolysin. The overall sequencing strategy evolved is assessed.

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Year: 1974 PMID： 4618777 PMCID： PMC1168175 DOI： 10.1042/bj1410701

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. Studies towards the complete sequence determination of proteins by mass spectrometry; a rapid procedure for the successful permethylation of histidine containing peptides.

Authors: H R. Morris
Journal: FEBS Lett Date: 1972-05-15 Impact factor: 4.124

2. Mass spectrometric sequence determination of permethylated peptide mixtures.

Authors: P Roepstorff; R K. Spear; K Brunfeldt
Journal: FEBS Lett Date: 1971-06-24 Impact factor: 4.124

3. Evidence for the amino acid sequence of porcine pancreatic elastase.

Authors: D M Shotton; B S Hartley
Journal: Biochem J Date: 1973-04 Impact factor: 3.857

4. Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure.

Authors: S S Taylor; P W Rigby; B S Hartley
Journal: Biochem J Date: 1974-09 Impact factor: 3.857

5. Studies towards the complete sequence determination of proteins by mass spectrometry: derivatisation of methionine, cysteine and arginine containing peptides.

Authors: H R Morris; R J Dickinson; D H Williams
Journal: Biochem Biophys Res Commun Date: 1973-03-05 Impact factor: 3.575

6. Determination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry.

Authors: H R Morris; D H Williams; R P Ambler
Journal: Biochem J Date: 1971-11 Impact factor: 3.857

7. Mass spectrometry of N-methylated peptide derivatives.

Authors: K L Agarwal; R A Johnstone; G W Kenner; D S Millington; R C Sheppard
Journal: Nature Date: 1968-08-03 Impact factor: 49.962

8. Mass spectrometric determination of the amino-acid sequence of peptides.

Authors: M M Shemyakin; Y A Ovchinnikov; A A Kiryushkin; E I Vinogradova; A I Miroshnikov; Y B Alakhov; V M Lipkin; Y B Shvetsov; N S Wulfson; B V Rosinov; V N Bochkarev; V M Burikov
Journal: Nature Date: 1966-07-23 Impact factor: 49.962

9. Mass-spectrometric determination of the amino acid sequences in peptides isolated from the protein silk fibroin of Bombyx mori.

Authors: F Lucas; M Barber; W A Wolstenholme; A J Geddes; G N Graham; H R Morris
Journal: Biochem J Date: 1969-10 Impact factor: 3.857

10. Dihydrofolate reductase: low-resolution mass-spectrometric analysis of an elastase digest as a sequencing tool.

Authors: H R Morris; K E Batley; N C Harding; R A Bjur; J G Dann; R W King
Journal: Biochem J Date: 1974-02 Impact factor: 3.857

14 in total

1. Sequence similarity between Pseudomonas dihydrodiol dehydrogenase, part of the gene cluster that metabolizes polychlorinated biphenyls, and dehydrogenases involved in metabolism of ribitol and glucitol and synthesis of antibiotics and 17 beta-oestradiol, testosterone and corticosterone.

Authors: M E Baker
Journal: Biochem J Date: 1990-05-01 Impact factor: 3.857

2. Appendix: The sequence of the blocked N-terminal peptide from Neurospora glutamate dehydrogenase.

Authors: H R Morris; A Dell
Journal: Biochem J Date: 1975-09 Impact factor: 3.857

3. Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family.

Authors: D Ghosh; C M Weeks; P Grochulski; W L Duax; M Erman; R L Rimsay; J C Orr
Journal: Proc Natl Acad Sci U S A Date: 1991-11-15 Impact factor: 11.205

4. Structure of the Hordeum vulgare gene encoding dihydroflavonol-4-reductase and molecular analysis of ant18 mutants blocked in flavonoid synthesis.

Authors: K N Kristiansen; W Rohde
Journal: Mol Gen Genet Date: 1991-11

5. Mass-spectrometric identification and sequence location of the ten residues of the new amino acid (gamma-Carboxyglutamic acid) in the N-terminal region of prothrombin.

Authors: H R Morris; A Dell
Journal: Biochem J Date: 1976-03-01 Impact factor: 3.857

6. Ribitol dehydrogenase of Klebsiella aerogenes. Sequence of the structural gene.

Authors: T Loviny; P M Norton; B S Hartley
Journal: Biochem J Date: 1985-09-15 Impact factor: 3.857

7. Ribitol dehydrogenase of Klebsiella aerogenes. Sequence and properties of wild-type and mutant strains.

Authors: J M Dothie; J R Giglio; C B Moore; S S Taylor; B S Hartley
Journal: Biochem J Date: 1985-09-15 Impact factor: 3.857

8. Construction of intergeneric hybrids using bacteriophage P1CM: transfer of the Klebsiella aerogenes ribitol dehydrogenase gene to Escherichia coli.

Authors: P W Rigby; M J Gething; B S Hartley
Journal: J Bacteriol Date: 1976-02 Impact factor: 3.490

9. The N-terminal sequences of blood coagulation factor X1 and X2 light chains. Mass-spectrometric identification of twelve residues of gamma-carboxyglutamic acid in their vitamin K-dependent domains.

Authors: H C Thøgersen; T E Petersen; L Sottrup-Jensen; S Magnusson; H R Morris
Journal: Biochem J Date: 1978-11-01 Impact factor: 3.857

10. Discovery and characterization of a new transposable element, Tn4811, in Streptomyces lividans 66.

Authors: C W Chen; T W Yu; H M Chung; C F Chou
Journal: J Bacteriol Date: 1992-12 Impact factor: 3.490