Literature DB >> 4207389

Dihydrofolate reductase: low-resolution mass-spectrometric analysis of an elastase digest as a sequencing tool.

H R Morris, K E Batley, N C Harding, R A Bjur, J G Dann, R W King.   

Abstract

An elastase digest of a protein of unknown structure, dihydrofolate reductase, was studied by mass spectrometry. This soluble digest contained a large number of small peptides in different yields, within the ideal molecular-weight range (200-1200) for mixture-analysis mass spectrometry. Sequences of the major component peptides in the digest are reported.

Entities:  

Mesh：

Substances：

Year:  1974        PMID： 4207389      PMCID： PMC1166130          DOI： 10.1042/bj1370409

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  The use of deuterated derivatives in peptide mixture analysis by mass spectrometry.

Authors:  P Roepstorff; K Brunfeldt
Journal:  FEBS Lett       Date:  1972-04-01       Impact factor: 4.124

2.  Studies towards the complete sequence determination of proteins by mass spectrometry; a rapid procedure for the successful permethylation of histidine containing peptides.

Authors:  H R. Morris
Journal:  FEBS Lett       Date:  1972-05-15       Impact factor: 4.124

3.  Mass spectrometric sequence determination of permethylated peptide mixtures.

Authors:  P Roepstorff; R K. Spear; K Brunfeldt
Journal:  FEBS Lett       Date:  1971-06-24       Impact factor: 4.124

4.  THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.

Authors:  R P AMBLER
Journal:  Biochem J       Date:  1963-11       Impact factor: 3.857

5.  Evidence for the amino acid sequence of porcine pancreatic elastase.

Authors:  D M Shotton; B S Hartley
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

6.  Studies towards the complete sequence determination of proteins by mass spectrometry: derivatisation of methionine, cysteine and arginine containing peptides.

Authors:  H R Morris; R J Dickinson; D H Williams
Journal:  Biochem Biophys Res Commun       Date:  1973-03-05       Impact factor: 3.575

7.  Determination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry.

Authors:  H R Morris; D H Williams; R P Ambler
Journal:  Biochem J       Date:  1971-11       Impact factor: 3.857

8.  Determination of amino acid sequences in peptide mixtures by mass spectrometry.

Authors:  F W McLafferty; R Venkataraghavan; P Irving
Journal:  Biochem Biophys Res Commun       Date:  1970-04-24       Impact factor: 3.575

9.  Affinity chromatography of dihydrofolate reductase.

Authors:  P C Newbold; N G Harding
Journal:  Biochem J       Date:  1971-08       Impact factor: 3.857

10.  Mass-spectrometric determination of the amino acid sequences in peptides isolated from the protein silk fibroin of Bombyx mori.

Authors:  F Lucas; M Barber; W A Wolstenholme; A J Geddes; G N Graham; H R Morris
Journal:  Biochem J       Date:  1969-10       Impact factor: 3.857
  10 in total
  3 in total

1.  Appendix: The sequence of the blocked N-terminal peptide from Neurospora glutamate dehydrogenase.

Authors:  H R Morris; A Dell
Journal:  Biochem J       Date:  1975-09       Impact factor: 3.857

2.  Elastase digests: new ammunition for shotgun membrane proteomics.

Authors:  Benjamin Rietschel; Tabiwang N Arrey; Bjoern Meyer; Sandra Bornemann; Malte Schuerken; Michael Karas; Ansgar Poetsch
Journal:  Mol Cell Proteomics       Date:  2008-12-30       Impact factor: 5.911

3.  A mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from Klebsiella aerogenes.

Authors:  H R Morris; D H Williams; G G Midwinter; B S Hartley
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.