Literature DB >> 4615303

Isolation and chromatographic behaviour of phenylalanine tRNA from barley embryos.

D Labuda, Z Janowicz, T Haertlé, J Augustyniak.   

Abstract

Two fractions of phenylalanine tRNA (tRNA(Phe) (1) and tRNA(Phe) (2)) were purified by BD-cellulose and RPC-5 chromatography of crude tRNA isolated from barley embryos. Successive RPC-5 rechromatography runs of tRNA(Phe) (2) showed its conversion into more stable tRNA(Phe) (1), suggesting that the two fractions have essentially the same primary structure. Both tRNA(Phe) (1) and tRNA(Phe) (2) had about the same acceptor activity, but tRNA(Phe) (2) was aminoacylated much faster than tRNA(Phe) (1). RPC-5 chromatography of crude aminoacylated tRNA showed higher contents of phe-tRNA(Phe) (2) than of phe-tRNA(Phe) (1) but the ratio of these two fractions estimated by relative fluorescence intensity was about 1. Fluorescence spectra of tRNA(Phe) from barley embryos suggest that it contains Y base similar to Y(w) from wheat tRNA(Phe).

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Year:  1974        PMID： 4615303      PMCID： PMC343449          DOI： 10.1093/nar/1.12.1703

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Isolation and structure determination of the fluorescent base from bovine liver phenylalanine transfer ribonucleic acid.

Authors:  S H Blobstein; D Grunberger; I B Weinstein; K Nakanishi
Journal:  Biochemistry       Date:  1973-01-16       Impact factor: 3.162

2.  Comparative studies on mammalian and yeast phenylalanine transfer ribonucleic acids.

Authors:  L M Fink; K W Lanks; T Goto; I B Weinstein
Journal:  Biochemistry       Date:  1971-05-11       Impact factor: 3.162

3.  Yeast phenylalanyl transfer ribonucleic acid synthetase. Purification, molecular weight, and subunit structure.

Authors:  J Schmidt; R Wang; S Stanfield; B R Reid
Journal:  Biochemistry       Date:  1971-08-17       Impact factor: 3.162

4.  Studies on polynucleotides. LXXXII. Yeast phenylalanine transfer ribonucleic acid: partial digestion with ribonuclease T-1 and derivation of the total primary structure.

Authors:  U L RajBhandary; S H Chang
Journal:  J Biol Chem       Date:  1968-02-10       Impact factor: 5.157

5.  The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

Authors:  U E Loening
Journal:  Biochem J       Date:  1967-01       Impact factor: 3.857

6.  Primary structure of wheat germ phenylalanine transfer RNA.

Authors:  B S Dudock; G Katz; E K Taylor; R W Holley
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

7.  Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.

Authors:  R L Pearson; J F Weiss; A D Kelmers
Journal:  Biochim Biophys Acta       Date:  1971-02-11

8.  Chemical modification of the fluorescent base in phenylalanine transfer ribonucleic acid.

Authors:  D Yoshikami; E B Keller
Journal:  Biochemistry       Date:  1971-07-20       Impact factor: 3.162

9.  The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.

Authors:  I Gillam; S Millward; D Blew; M von Tigerstrom; E Wimmer; G M Tener
Journal:  Biochemistry       Date:  1967-10       Impact factor: 3.162

10.  Evidence for the absence of the terminal adenine nucleotide at the amino acid-acceptor end of transfer ribonucleic acid in non-lactating bovine mammary gland and its inhibitory effect on the aminoacylation of rat liver transfer ribonucleic acid.

Authors:  M D Herrington; A O Hawtrey
Journal:  Biochem J       Date:  1970-02       Impact factor: 3.857
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  1 in total

1.  Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum.

Authors:  P Cornelis; E Classen; J Claessen
Journal:  Nucleic Acids Res       Date:  1975-07       Impact factor: 16.971
  1 in total

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