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Literature DB >> 1153333

Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum.

Abstract

RPC 5 (Reversed Phase Chromatography) of aminoacyl-tRNA's from healthy and crown gall (induced by Agrobacterium tume-faciens strain B6) tobacco tissues were compared for eleven amino acids. For ten amino acids: alanine, arginine, glutamic acid, glycine, isoleucine, leucine, lysine, methionine, tyrosine, and valine, no qualitative or quantitative differences could be detected between aminoacyl-tRNA's from both sources. Phenylalanyl-tRNA's from crown gall tissues gave two peaks on RPC 5; the minor early eluting species (peak 1) was always absent in elution profiles of phenylalanyl-tRNA's from healthy tissues or from tobacco leaves. After the "Y" base was removed by pH 2.9 treatment, peak 2 of phenylalanine tRNA was shifted to the position of peak 1.

Entities: Chemical Species

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Year: 1975 PMID： 1153333 PMCID： PMC343502 DOI： 10.1093/nar/2.7.1153

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA.

Authors: U L Rajbhandary; S H Chang; A Stuart; R D Faulkner; R M Hoskinson; H G Khorana
Journal: Proc Natl Acad Sci U S A Date: 1967-03 Impact factor: 11.205

2. The primary structure of two mammalian tRNAs Phe: identity of calf liver and rabbit liver tRNAs Phe.

Authors: G Keith; J P Ebel; G Dirheimer
Journal: FEBS Lett Date: 1974-11-01 Impact factor: 4.124

3. Purification and properties of the major phenylalanyl transfer RNA species in drug-resistant Ehrlich tumor cells.

Authors: M Hayashi; A C Griffin
Journal: Cancer Res Date: 1974-12 Impact factor: 12.701

4. Phenylalanine transfer RNA species in early development of barley.

Authors: V S Hiatt; L A Snyder
Journal: Biochim Biophys Acta Date: 1973-09-28

5. An extra species of lysine transfer ribonucleic acid in polyoma virus-transformed cells in tissue culture.

Authors: E L Jacobson; H Juarez; C Hedgcoth; R A Consigli
Journal: Arch Biochem Biophys Date: 1974-08 Impact factor: 4.013

6. Elevated levels of acceptor activity of hepatoma transfer ribonucleic acid.

Authors: A J Ouellette; M W Taylor
Journal: Biochemistry Date: 1973-08-28 Impact factor: 3.162

Review 7. Regulation of tRNA.

Authors: U Z Littauer; H Inouye
Journal: Annu Rev Biochem Date: 1973 Impact factor: 23.643

Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum.

1. Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA.

2. The primary structure of two mammalian tRNAs Phe: identity of calf liver and rabbit liver tRNAs Phe.

3. Purification and properties of the major phenylalanyl transfer RNA species in drug-resistant Ehrlich tumor cells.

4. Phenylalanine transfer RNA species in early development of barley.

5. An extra species of lysine transfer ribonucleic acid in polyoma virus-transformed cells in tissue culture.

6. Elevated levels of acceptor activity of hepatoma transfer ribonucleic acid.

Review 7. Regulation of tRNA.

8. Characterization and fractionation of tobacco leaf transfer RNA.

9. Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids.

10. Isolation and chromatographic behaviour of phenylalanine tRNA from barley embryos.

1. Functionally impaired tRNA from ethionine treated rats as detected in injected Xenopus oocytes.